pLX-WMoV vector (V004875)

MSDS
Basic Vector Information

Vector Name:: pLX-WMoV
Antibiotic Resistance:: Kanamycin
Length:: 10508 bp
Type:: Binary vector
Replication origin:: pBBR1 oriV
Host:: Plants
Source/Author:: Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, Chen YR.
Promoter:: CaMV 35S (enhanced)
pLX-WMoV vector Vector Map

View Map in NovoBuilder
Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-WMoV vector Sequence

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Download GeneBank File(.gb)
LOCUS       40924_29441       10508 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-WMoV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 10508)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol. 
            Methods (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2018) Agricultural Biotechnology Research Center, Academia 
            Sinica, 128 Academia Road, Section 2, Nangang District, Taipei, 
            Taipei 11529, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1203
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1204..7500
                     /label=Wasabi mottle virus
                     /note="Wasabi mottle virus"
     CDS             1269..6062
                     /codon_start=1
                     /transl_except="(pos: 4578 .. 4580,  aa: OTHER)"
                     /product="RNA replicase readthrough protein"
                     /label=RNA replicase readthrough protein
                     /protein_id="AYC81452.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ*QLQ
                     IDAVFKGVNLNVPTPKSGDWRDMQFYYDTLLPGNSTILNEFDAVTMNLRDISLNVKDCR
                     IDFSKSVQVPKEKPVFLKPKIRTAAEMPRTAGLIENLVAMIKRNMNAPDLTGTIDIEDT
                     ASLVVEKFWDSYIIKEFSGIEGMARTRQGFSRWLSKQESSTVGQLADFNFVDLPAVDEY
                     KHMIKSQPKQKLDLSIQDEYPALQTIVYHSKKINAIFGPMFSELTRMLLERVDTSKFLF
                     YTRKTPTQIEEFFSDLDSSQAMEILELDISKYDKSQNEFHCAVEYKIWEKLGIDEWLAE
                     VWRQGHRKTTLKDYTAGIKTCLWYQRKSGDVTTFIGNTIIIAACLSSMIPMEKVIKAAF
                     CGDDSLIYIPKGLDLPDIQAGANLTWNFEAKLFRKKYGYFCGRYVIHHDRGAIVYYDPL
                     KLISKLGCKHIKDEVHLEELRRSLCDVASNLNNCAYFSQLDEAVAEVHKTAVGGSFAYC
                     SIIKYLSDKRLFKDLFFV"
     CDS             1269..4580
                     /codon_start=1
                     /product="RNA replicase"
                     /label=RNA replicase
                     /protein_id="AYC81451.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ"
     CDS             6065..6868
                     /codon_start=1
                     /product="movement protein"
                     /label=movement protein
                     /protein_id="AYC81453.1"
                     /translation="MSTVSYKPKVSDFLALSRTEEILPKALTRLKTVSISTKDVISVKE
                     SESLCDIDLLVNVPLDKFRYVGILGVVFTGEWLIPDFVKGGVTVSVIDKRLENSKECII
                     GTYRAAAKDRRFQFKLVPNYFVSVTDAKRKPWQVHVRIQNLKIEAGWQPLALEVVSVAM
                     VTNNVVVKGLRERVIAVDDPNVEGFEGVVDEFIDSVAAFKAVDNFRKKKRKIGGRDVIS
                     KYKYRPEKYAGPDSLLNKEENVIQHHELESVPVFRSGMGGAHSNA"
     CDS             6792..7265
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81454.1"
                     /translation="MSYNITNSNQYQFFAAVWAEPIAMLNQCVSALSQSYQTQAARDTV
                     RQQFSNLLSAIVTPNQRFPETGYRVYVNSAVLKPLYEALMKSFDTKNRIIETEEESRPS
                     ASEVANATQRVDDATVAIRSQIQLLLSELSSGHGLMNRAEFEVLIPWATAPAK"
     misc_feature    7501..7546
                     /label=ribozyme
                     /note="ribozyme"
     terminator      7557..7809
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7874..7897
                     /label=RB
                     /note="RB"
     regulatory      7962..8002
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(8049..8861)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(8990..9649)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(9650..10421)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
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