LOCUS       40924_29441       10508 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-WMoV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 10508)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol. 
            Methods (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2018) Agricultural Biotechnology Research Center, Academia 
            Sinica, 128 Academia Road, Section 2, Nangang District, Taipei, 
            Taipei 11529, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1203
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1204..7500
                     /label=Wasabi mottle virus
                     /note="Wasabi mottle virus"
     CDS             1269..6062
                     /codon_start=1
                     /transl_except="(pos: 4578 .. 4580,  aa: OTHER)"
                     /product="RNA replicase readthrough protein"
                     /label=RNA replicase readthrough protein
                     /protein_id="AYC81452.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ*QLQ
                     IDAVFKGVNLNVPTPKSGDWRDMQFYYDTLLPGNSTILNEFDAVTMNLRDISLNVKDCR
                     IDFSKSVQVPKEKPVFLKPKIRTAAEMPRTAGLIENLVAMIKRNMNAPDLTGTIDIEDT
                     ASLVVEKFWDSYIIKEFSGIEGMARTRQGFSRWLSKQESSTVGQLADFNFVDLPAVDEY
                     KHMIKSQPKQKLDLSIQDEYPALQTIVYHSKKINAIFGPMFSELTRMLLERVDTSKFLF
                     YTRKTPTQIEEFFSDLDSSQAMEILELDISKYDKSQNEFHCAVEYKIWEKLGIDEWLAE
                     VWRQGHRKTTLKDYTAGIKTCLWYQRKSGDVTTFIGNTIIIAACLSSMIPMEKVIKAAF
                     CGDDSLIYIPKGLDLPDIQAGANLTWNFEAKLFRKKYGYFCGRYVIHHDRGAIVYYDPL
                     KLISKLGCKHIKDEVHLEELRRSLCDVASNLNNCAYFSQLDEAVAEVHKTAVGGSFAYC
                     SIIKYLSDKRLFKDLFFV"
     CDS             1269..4580
                     /codon_start=1
                     /product="RNA replicase"
                     /label=RNA replicase
                     /protein_id="AYC81451.1"
                     /translation="MAQFQQTIDMQTLQAAAGRNSLVNDLASRRVYDNAVEELNARSRR
                     PKVHFSKSVSTEQTLLASNAYPEFEISFTHTQHAVHSLAGGLRSLELEYLMMQVPFGSL
                     TYDIGGNFAAHLFKGRDYVHCCMPNLDVRDIARHEGHKEAVHGYISRLKRQRRPVPEYQ
                     RAAFNNYSENPHFVHCDQPFQQCALSTVNGDDTYAIALHSVYDIPVEEFGGALLRKNVK
                     VCYAAFHFHENMLLDCDTVTLEEIGATFQRAGDKLNFFFHNESTLNYTHSFSNIIKYVC
                     KTFFPASQRFVYHKEFLVTRVNTWYCKFTRVDTFTLFRGVYKNSVDSDEFYKAMDDAWE
                     YKKTLAMLNAERTIFKDNAAINFWFPKVRDMVIVPLFDASITTGRMSRREVMVNKDFVY
                     TVLNHIKTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDTDKAILGPLAMTFFLVT
                     KLGHVQDEIVLKKFQKFDSTAKELIWTSLCDALMGVIPSVKETLARGGFVKLAEESLEI
                     KIPELYCTFTDRLVLEYKRAEEFKSCDLSKPLEESEKYYNALSELSVLENLDSFDLEAF
                     KELCQKKSVDPDVAAKVVVAIMKSELTLPFKKPTEEEIAESLSNDTIRNEGLSLSNTAP
                     FPCVSNLEGGLVPACGLCPKSGGFDKIDMDISEFHLMSVDAVKKGAMMSAVYTGNIKVQ
                     QMKNFVDYLSASLSATVSNLCKVLRDVHGVDPESQEKSGVWDVRRGRWLLKPNAKCHAW
                     GVAEDANHKLVIVLLNWDDGKPVCDQTWYRLAVSSDSLVYSDIGKLKTLTSCCVDGEPP
                     EPKAKVVLVDGVPGCGKTKEILEKVNFSEDLVLVPGKEASKMIIRRANQAGVTRADRDN
                     VRTVDSFLMHPPKRVFKRLFIDEGLMLHTGCVNFLTLISQCDIAYVYGDTQQIPFICRV
                     ANFPYPKHFARLVVDEKEDRRITLRCPADVTFFLNKKYDGSVLCTSSVERSVSAQVVRG
                     KGALNPITLPLEGKILTFTQADKFELLDKGYKDVNTVHEVQGETYEKTAIVRLTATPLE
                     IISRASPHVLVALTRHTTSCKYFSVVLDPLVSVISEMEKLSNFILDMYKVESGTQ"
     CDS             6065..6868
                     /codon_start=1
                     /product="movement protein"
                     /label=movement protein
                     /protein_id="AYC81453.1"
                     /translation="MSTVSYKPKVSDFLALSRTEEILPKALTRLKTVSISTKDVISVKE
                     SESLCDIDLLVNVPLDKFRYVGILGVVFTGEWLIPDFVKGGVTVSVIDKRLENSKECII
                     GTYRAAAKDRRFQFKLVPNYFVSVTDAKRKPWQVHVRIQNLKIEAGWQPLALEVVSVAM
                     VTNNVVVKGLRERVIAVDDPNVEGFEGVVDEFIDSVAAFKAVDNFRKKKRKIGGRDVIS
                     KYKYRPEKYAGPDSLLNKEENVIQHHELESVPVFRSGMGGAHSNA"
     CDS             6792..7265
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81454.1"
                     /translation="MSYNITNSNQYQFFAAVWAEPIAMLNQCVSALSQSYQTQAARDTV
                     RQQFSNLLSAIVTPNQRFPETGYRVYVNSAVLKPLYEALMKSFDTKNRIIETEEESRPS
                     ASEVANATQRVDDATVAIRSQIQLLLSELSSGHGLMNRAEFEVLIPWATAPAK"
     misc_feature    7501..7546
                     /label=ribozyme
                     /note="ribozyme"
     terminator      7557..7809
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7874..7897
                     /label=RB
                     /note="RB"
     regulatory      7962..8002
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(8049..8861)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(8990..9649)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(9650..10421)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcac cagtacgcac gattcaaggc ttgcttcaca aaccaaggca
      301 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
      361 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
      421 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
      481 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      541 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      601 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      661 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      721 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      781 atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
      841 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      901 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      961 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatc ctctgccgac
     1021 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     1081 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     1141 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     1201 agggtttaaa ttattgcaac aacaacaaca aattacaata ataacaaaca aatacaaaca
     1261 acaacaacat ggcacagttt caacaaacaa ttgacatgca gacacttcag gctgcagcgg
     1321 gacgtaacag cctggttaac gatttagcct ctcgacgtgt ttacgataac gcggtcgagg
     1381 agttaaatgc gcgttcaaga cgtcctaagg ttcatttctc caagtcggtg tcgacggaac
     1441 agacactact agcttcaaac gcatatccgg agtttgagat ttccttcact catacccaac
     1501 atgccgtaca ttcacttgcc ggcgggttgc ggtctcttga gctggaatat ctcatgatgc
     1561 aagttccttt cggatctctg acgtatgata tcggcggtaa ctttgctgcg caccttttta
     1621 agggtcgtga ctacgtgcac tgctgtatgc caaatttgga cgttcgcgat atagctcgcc
     1681 atgaaggaca taaggaagcc gttcacggct acatctcccg cttgaaaagg caaaggagac
     1741 ctgttcctga gtatcagaga gctgcgttca acaactactc ggagaatccg catttcgttc
     1801 attgtgacca accttttcaa cagtgtgcct taagtactgt gaacggtgat gacacttatg
     1861 ctattgccct tcacagtgta tacgacattc ctgttgagga gttcggtggt gcgctactca
     1921 ggaagaacgt caaagtttgt tatgcggctt tccatttcca tgagaatatg ctactcgatt
     1981 gtgacacagt cactcttgag gagattggag ctacatttca acgtgcaggc gacaagttaa
     2041 actttttctt ccacaatgag agcactctca actataccca cagttttagt aatataatta
     2101 agtatgtgtg taaaaccttt tttcccgcca gtcagcggtt tgtgtatcat aaggagtttt
     2161 tagttacaag agtcaatacg tggtattgta agttcactag agtggacact tttactcttt
     2221 ttcgtggtgt gtacaaaaac agtgtcgata gcgatgagtt ttataaggct atggatgacg
     2281 catgggaata caagaagact ttagctatgt taaatgccga gaggactatc ttcaaggata
     2341 acgcggctat taatttttgg tttccgaagg tcagagatat ggtcatcgtt cctcttttcg
     2401 acgcatccat cacaacagga aggatgtcta gaagagaagt tatggtgaat aaggattttg
     2461 tttatacagt cctcaaccat attaaaacat atcaggcaaa ggctttgact tacgctaacg
     2521 tactgtcctt tgtagagtct atcaggtcga gagtcataat taatggggtc acagctaggt
     2581 ctgagtggga tacggataag gcaattctag gtccgttagc aatgactttt ttccttgtta
     2641 caaagttagg tcatgtccag gatgaaatag tcctgaagaa gttccaaaag tttgattcga
     2701 ccgccaaaga gctgatttgg actagtctct gcgatgccct gatgggggtt ataccctcgg
     2761 tcaaagagac gttggcacgt ggtggttttg tcaaactcgc tgaagagagt ttggagatca
     2821 agattccaga actgtattgc actttcacag acaggttagt gctggagtac aaaagagctg
     2881 aagagttcaa gtcttgtgat ctgtctaaac cgttagaaga gtcagagaaa tactacaacg
     2941 ctttatccga gctgtctgtg ctcgagaacc tcgactcttt tgacttggaa gcctttaaag
     3001 agttgtgcca gaagaaaagc gttgatccgg atgtcgctgc aaaggtggtg gtggcgataa
     3061 tgaagagtga gttgacgtta ccgttcaaga agcctactga ggaagaaatc gcagagtcgc
     3121 ttagcaatga tacaatacgg aatgagggtt tgagtctgag taataccgca cctttcccgt
     3181 gtgtgagcaa tctcgaggga gggttggtgc cagcgtgcgg gctgtgtccg aaaagtggtg
     3241 gtttcgacaa gattgatatg gatatatctg agttccatct catgagtgta gatgcagtta
     3301 aaaaaggggc tatgatgtca gcggtgtaca cagggaacat caaggttcag cagatgaaga
     3361 actttgtaga ttatctaagt gcgtcactgt cagctactgt ctcaaatctc tgtaaggtgc
     3421 ttagagatgt tcatggggta gatccagagt cacaggagaa atccggagtg tgggatgtga
     3481 gaagaggacg atggttacta aaacctaacg cgaaatgtca cgcgtgggga gttgcggaag
     3541 atgccaatca taagttggtt atcgtgttac ttaactggga tgatggaaaa cctgtctgcg
     3601 atcagacatg gtatcgattg gcggtgtcta gtgattcttt ggtgtattcc gatattggga
     3661 aacttaaaac gttgacgtcg tgctgtgtgg atggcgaacc tccagagcct aaggccaagg
     3721 tggttcttgt tgatggagtt ccaggatgtg gaaaaacgaa ggaaattctg gagaaggtta
     3781 acttctctga agatttggtt ttagtccctg gaaaggaggc gtcgaagatg attatacgac
     3841 gagcgaacca ggcaggagtt acgagagctg atagggataa cgtgagaaca gtggattctt
     3901 tcttaatgca ccctccaaag agggtattta agaggttgtt cattgatgaa ggattaatgc
     3961 tgcataccgg ttgtgttaac tttttgacgc tgatttcgca atgcgatata gcatacgtct
     4021 acggcgacac acaacagatt cccttcattt gtagggttgc aaattttccg tatcccaaac
     4081 attttgcaag acttgtcgtt gatgaaaagg aagacagaag gataacgctc agatgccctg
     4141 cagacgtcac ttttttcctt aacaagaaat atgacggctc agtgttgtgt accagcagtg
     4201 tggaaagatc ggtcagtgcg caggttgtga gagggaaagg tgctttaaac ccgataactt
     4261 taccgttaga ggggaaaatt ttaaccttca cacaggccga caagttcgag ttactagaca
     4321 aaggctacaa ggatgtgaat acggtccatg aggttcaagg ggaaacatat gaaaaaacag
     4381 ctattgttcg gttaacggca actccgttgg agatcatatc cagggcgtca cctcatgttt
     4441 tggtggcgct gacgagacac acgactagtt gtaaatattt ttcggtcgtg ttggatcctt
     4501 tagtgagtgt gatttcagag atggagaagt tgtccaattt catccttgac atgtacaagg
     4561 tggagtcggg aacccaatag caattacaga tcgatgcggt gttcaaaggg gtcaacttaa
     4621 atgttccgac gccaaagtca ggagattgga gagacatgca gttttattac gacactcttc
     4681 ttcctggtaa cagcactatt cttaacgaat ttgatgctgt taccatgaat ttgagggata
     4741 tatccttaaa tgtcaaagat tgcagaatcg acttctctaa atccgtgcaa gtgcccaaag
     4801 aaaaaccagt tttcttaaaa cccaagataa gaactgcggc agaaatgccg agaactgcgg
     4861 ggttaataga gaatttggtt gcaatgatca agagaaatat gaatgcaccg gatttgactg
     4921 ggacgataga tatagaggac actgcatcac tggtggttga aaagttttgg gattcgtata
     4981 tcataaagga gttcagcgga attgagggta tggcaaggac aaggcaaggt ttttcaagat
     5041 ggctttctaa acaagagtcg tctacggtcg gtcagttagc ggatttcaac tttgtggatt
     5101 tgcctgcagt tgatgagtac aagcatatga tcaagagtca gcctaagcaa aagttagact
     5161 tgagtattca agatgaatat cctgctttgc agacgatagt ctaccattca aagaagatca
     5221 atgcaatttt tggtcctatg ttttcagaac ttacgagaat gcttcttgaa agagttgata
     5281 catctaagtt tctgttttac actaggaaaa cgccgactca gattgaggaa tttttctcag
     5341 acctcgattc gtcgcaagca atggaaattt tggaactcga catttcaaag tacgacaagt
     5401 ctcagaacga gttccattgt gctgtggaat acaagatatg ggagaagctt ggaatagacg
     5461 agtggttggc agaggtgtgg cgacaagggc acaggaaaac gacgctgaaa gactatacgg
     5521 ccgggatcaa aacgtgtttg tggtatcaga ggaaaagcgg tgatgtaact acctttattg
     5581 ggaacaccat catcatcgct gcgtgcttaa gttctatgat tccgatggaa aaggtgatta
     5641 aagcagcatt ctgcggtgac gacagtttga tttacattcc gaaaggttta gatctgccgg
     5701 atattcaagc tggagccaac ctaacgtgga atttcgaggc gaagctcttc aggaaaaagt
     5761 atggttattt ctgcggtcga tatgtaatcc accatgatag aggagctatt gtatattacg
     5821 atccacttaa gctaatatct aagttaggtt gtaaacatat aaaggatgag gtccacttag
     5881 aagagttacg taggtctctg tgtgatgtag ctagtaactt aaataattgt gcgtattttt
     5941 ctcaattaga tgaggccgtt gccgaggttc ataaaaccgc ggttggcgga tcgtttgctt
     6001 attgtagtat aattaaatac ttatcagata aaaggttgtt taaagatttg ttttttgttt
     6061 gataatgtcg accgtgtcgt acaagcctaa ggtgagtgac ttccttgctc tttcgagaac
     6121 agaggaaatt ttaccgaaag ctttaacgag attgaagact gtgtctataa gtactaagga
     6181 tgtcatatca gttaaggagt ctgagtcttt gtgtgatata gatttgttag ttaatgtgcc
     6241 attagataag tttagatacg tgggtatttt aggagttgtt tttactggcg agtggcttat
     6301 tccggatttc gttaagggtg gtgtgacggt gagtgtgatt gataagcgtc tggaaaactc
     6361 gaaggagtgt ataattggta cgtacagagc tgctgctaag gacagaaggt tccagtttaa
     6421 gctggtgcct aactactttg tgtctgtaac ggacgctaag agaaaaccgt ggcaggttca
     6481 tgtgcgtatt caaaatctga agattgaagc tggatggcaa ccgttggctc tggaagttgt
     6541 ttccgtagct atggttacca acaatgtggt tgtgaagggt ttaagagaaa gagtcatcgc
     6601 tgtagatgat ccgaacgttg agggtttcga aggtgtagtt gacgaattca tcgattcggt
     6661 tgcagcattt aaagcggttg acaatttcag gaaaaagaaa aggaagattg gaggaaggga
     6721 tgtgattagt aagtataagt atagaccaga gaagtacgcc ggtcctgatt cgttacttaa
     6781 taaagaagaa aatgtcatac aacatcacga actcgaatca gtaccagttt ttcgcagcgg
     6841 tatgggcgga gcccatagca atgcttaacc agtgcgtatc agcgttgtca cagtcgtatc
     6901 agacacaagc tgcgagagat actgtcagac agcaattctc gaacttgtta agtgcgattg
     6961 tgacaccgaa ccagcggttt ccagaaacag gataccgggt gtatgttaat tcagcagttc
     7021 taaaaccgtt gtacgaggct cttatgaagt cttttgatac taaaaatagg atcattgaga
     7081 ccgaagaaga gtcgcgtcct tcggcttccg aagtagccaa tgcaacacaa cgtgttgacg
     7141 atgcgaccgt ggccatcagg agtcaaattc agcttttgct gagtgagctt tccagtggac
     7201 atggtcttat gaacagggca gagtttgagg ttttaatacc ttgggctact gcgccggcta
     7261 aataggcgtg gtgcacacga tagtgcatag tgttttcctc tccacttaaa tcgaagaggt
     7321 attcttacgg cgtaaatccg taagggtggc gtaaaccaaa ttacgtaatg ttttgggttc
     7381 catttaaatc gaaacccgtt atttcctgga tcacctgtta acgcacgctt ggcgtgtatt
     7441 acagtgggaa taactaaaag tgagaggttc gaatcctccc taaccccggg taggggccca
     7501 gtcaccggat gtgctttccg gtctgatgag tccgtgagga cgaaactggg cctgcggatc
     7561 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     7621 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     7681 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     7741 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     7801 tactcgatct cgtcaggaga cgggacaagg atgcgagcta gcctgcagga attgttgatt
     7861 ttgtgatgac tgatggcagg atatatgcgg ttgtaattca tttttattgt ctaaatttct
     7921 gtatttgttt gtttgttcgg ttgtaaattt ttttggaaga acaagaaaag aaaaaacacc
     7981 cgttagggtg tttttagtta gtgtggcgcg ccgacttgcg acatgcggtc ctttgcaatc
     8041 aactattaga aaaattcatc cagcatcaga tgaaattgca gtttgttcat atccggatta
     8101 tcaatgccat atttctgaaa cagacgtttt tgcaggctcg ggctaaattc gcccaggcag
     8161 ttccacagaa tggccagatc ctgataacga tccgcaatgc ccacacggcc cacatcaatg
     8221 cagccaatca gtttgccttc atcgaaaatc aggttatcca ggctaaaatc gccgtgggtc
     8281 accacgctat ccgggctaaa cggcagcagt ttatgcattt ctttccacac ctgttccacc
     8341 ggccagccgt tacgttcatc atcaaaatcg ctcgcatcca ccaggccgtt gttcatacgg
     8401 ctctgcgcct gggccagacg aaacacacga tcgctgttaa acgggcagtt gcacaccgga
     8461 atgctatgca gacgacgcag aaacacggcc agcgcatcca caatgttttc gccgctatcc
     8521 ggatattctt ccagcacctg aaacgcggtt ttgcccggaa tcgcggtggt cagcagccac
     8581 gcatcatccg gggtgcgaat aaaatgttta atggtcggca gcggcataaa ttcggtcagc
     8641 cagttcagac gcaccatttc atcggtcaca tcgttcgcca cgctgccttt gccatgtttc
     8701 agaaacagtt ccggcgcatc cggtttgcca tacagacgat aaatggtcgc gccgctctga
     8761 cccacgttat cacgcgccca tttatagcca tacagatccg catccatgtt gctgttcaga
     8821 cgcggacggc tacagctcgt ttcacgctga atatggctca taacacccct tgtattactg
     8881 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     8941 catcagagat tttgagacac aagatcggat tggcggttat gcggttctac cggcgcggca
     9001 gcgttacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg
     9061 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca
     9121 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg
     9181 gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca
     9241 gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca
     9301 actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc
     9361 cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt
     9421 attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca
     9481 cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt
     9541 tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac
     9601 cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca
     9661 tgcggtaggg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag
     9721 cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta
     9781 cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt
     9841 tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag
     9901 ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc
     9961 ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc
    10021 ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc
    10081 ttcgactgcc cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct
    10141 gcgcggtcgc tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag
    10201 cgcgcaggcc gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa
    10261 ggccgcaggc cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg
    10321 gcaatccctg tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg
    10381 gttgtccacg ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat
    10441 atccacgggc tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc
    10501 gtaggggg
//
