Basic Vector Information
- Vector Name:
- pLX-UCBSV
- Antibiotic Resistance:
- Kanamycin
- Length:
- 13212 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Host:
- Plants
- Source/Author:
- Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.
- Promoter:
- CaMV 35S (enhanced)
pLX-UCBSV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-UCBSV vector Sequence
LOCUS 40924_29436 13212 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLX-UCBSV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13212)
AUTHORS Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez
D, Garcia JA.
TITLE Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors
with Compatible Replication Origins
JOURNAL ACS Synth Biol (2017) In press
PUBMED 28657330
REFERENCE 2 (bases 1 to 13212)
AUTHORS Pasin F, Garcia JA.
TITLE Direct Submission
JOURNAL Submitted (26-MAR-2017) Department of Plant Molecular Genetics,
Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid,
Madrid 28049, Spain
REFERENCE 3 (bases 1 to 13212)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13212)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2017) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-MAR-2017) Department of Plant Molecular Genetics, Centro
Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid
28049, Spain"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13212
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..24
/label=assembly linker
/note="assembly linker"
regulatory 25..72
/note="artificial; tested only in sense orientation"
/regulatory_class="terminator"
misc_feature 133..157
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
misc_feature 184..208
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 514..1183
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 1183..10275
/note="DSMZ: PV-0912; Ugandan cassava brown streak virus
isolate Ke_125"
CDS 1317..10025
/codon_start=1
/product="polyprotein"
/label=polyprotein
/protein_id="ASG92166.1"
/translation="MSTIQLFKTIRFGSFEPVKLDEGNNIIEKVPVDLLAGNDGSGPEE
QSEQKYHRKESGESWRKVTDLYSVIGNSVYCRSYVAMKNFLNDTKWGGLFKNKKGQELK
AAARLRRATSYGFMYDPVACAFECPVCRTKATALEAFTSDCDHCFEIKHIDDDRIVQTE
TKFYPINPIELDVEDNLVEAASLEWLKGDVEERVVDRVLLLEDKEIHVTKKALVKRRAE
TKLVANAADLTKKLTEICCESGIPIIDIDNSKRKAIPMVKLKHIFGKVECDDMFEEDRY
FLEHSNAGKIFRSCEKITYRMIRPGWSGAIILRDNVQSEDHDKFDFINDICVVQGKNLI
SYKIENAMRVKTETELDLIDLYSFNLSWAKSKDKFIKHFESDTTQLVRSCCTPSTLWLY
ARKARFYKFVDHMILKGSPIIDILVKMEYVGKHLDMFNSVEDVCTEYSHFMKELISETV
NDKSDPDVLRVRNLIRAHFESVLEYNKYELIDRIIEKKTQLEAQEIMSRELIKHQYGEL
FSWRERLCLKLGIGSSNLYTYWVEREEKQSEKSNVISCIISKPGIEMLINWVSEVCKSK
YHSLVNCVDSGILFLWSRIVHLARVSVYGYWNLWFRQAMCVLFIFLVSNFSGKIVSYLK
KLIVSEKKLAIKNEEGFVEVQGRKEESFVLKWCAAATLFLSFLNYDWAVGCVSAIGKMK
TIFSALGPDFIEKQDGDDDLKFTTFEVEIPGDSRSSSAQTFGEWIEHCIKFNLVSIEPT
TSGPMLTLERGKANELADQINCLNATDIRVHGGVGTGKSTALPYELIRYGAVLVCVPTR
VLANALHESFMSLFGFDVSLAYRGRVRTGSKPITIMTYGYALNHFHHNPRNLAQFQFII
MDEVHTFPVHLNPLFSLLRELSPDKKIIKTSATHVGHSVDLSTNHKVDIHTLEIMDVKK
WAELQGTSVFGDVTKEPGNVLVFVASYRDVDVCSDKLKDKGFPVIKVDGRNFRKNTEVQ
KMVDGMQGEVKFIVATNIIENGVTLDVDVVVDFGERISPNLCSEDRCILMQRQRISQAE
RKQRFGRVGRMKRGSVYKFGRETLPDSMRNRVGSTESALICFAYGLKPVVDDVDIGSVR
SVTQRQALTASMFEANYIFTAHLVDKQGFMPRPVFELMKSLLLHTDAVGVSSTYLATNM
SGWRRLKEYIRIDDNSRHVQEVQIPWYCSDMSDDFIVKLTECVKAAKPKSQCGYKVDNV
DFHTVAHKISVGESNIDESRALVATILDEVKQWRDGITYHSSTPRNKSLMSLMVGWIPR
KAEKTKEILDNRIQRLELLLNQLNGVRGIDDYESLVRFFSENPHSAEYLESQCASDYIE
EKVMSVKRNYDKSLILGMVGLAVATGTFAYWYMRRSAAVELVEKQAKHKYNRDKRTGRL
MFDMDDRETYENFGPEYTDDVISAKMTKAQKERDSKKKGWKAGKINRPMRVFHQLYGVN
PLEFDEVVMRVGKLETEPWDVKELNVDAMMIELDDDYHILRDDRMFGKKVSLAFKKEGA
DEETIVNLTPHRSKMTSSMSLAPMGFPEEEGRWRQTGAPLIRKIEKEDEVEVQVAKPES
TNPYDHILVRLGRAHLGTRVLNCFFHGSKCVIPYHLAEKGDREESLVIATTRGQFDFGP
MKNIKCRKVTDYDITICPLPNDVQPFRSKIVMREPKLGEEVVIVCFTRINGKIVMKVSD
KSTTYPAGGQFAHLWAYKYDGQPGDCGGPIVATVDQKVVGFHSGVIRNSREEKLRAVYT
PVNQELLNCISGDIQMTDFWTFNPDLVEWNSVARVSTFFPMTKAINTITVQANEGEELI
DGNLMIVGYVNREVYHNHVIKGKRESFMRYCEQFPNCAFTKELRDQYLPSILSKPAFRK
GLLKYNEPVRVGSVNFSCLIRAYLKVEEMFEDLGFLEEAGPQWDPIEILDDLNKKAAMG
ALYQGKKQDWLKSIEPADFITAVRESFKHLAGGDVGIWSGSLKAELRPVEKVLEQKTRV
FTGAPIDLLLGGKILVDNFNHFFYFNHLKGPWTVGINKFNKGWDRLARYFNHSWNFIDC
DGSRFDTSLAPILFQLVCHMREKFGNFDDIERAALRNLYTQIVYTPILTIDGYITKKHR
GNNSGQPSTVVDNTIILMIVVEYCKAVMESEGRVMQFKYMCNGDDLILNVPDDEVSIVQ
SRFRELFSECGLDYNFDDVHKSIETIEYMSHSFMLKDGVYIPKLKKERIVAILEWERGD
EIMRTRSALNAAYIESYGYDDLMVEIERYAVFWATDKGCEYPLLDRKRVEGLYKDDYTD
INEEWLIGILPPSFEHCYVDTQTKDLRGREKLELRIESHDRTLQMQMKFPVTFVTGNLG
KLAEVKSILGIANDVMAKNIDLPEVQGTPEEIVRKKAQLAVKMTNSPVLVEDTCLCFNA
FNGLPGPYIKWFLKELGLDGVVKMLSAFGDKSAYALCTFAYVHNESSDPIVFKGVVNGE
IVPPRGNNGFGWDPIFKPDGCGCTFAEMPSSIKNDFSHRRRALEKVKLFLDNLMVKQEK
NKASVALTIDVQALNQEEIEAEITALKKLWKDNGPTRTRSSFEARRLRAPQVERVNELL
QKLKDEGLQTKKRPCGEPDDGEVVDDDSNDGDNQRSEKEVVDESQNNQQVDPRKLKFKI
RGDGNAIRRDDIDKIPTNALEIKKTFKPPKVSQSAYIWIPRSQRDNLTPDVIQNFLAYI
PPSHAIDNQLASGIEVENWAIEVAKAYGVNIQEFYRTVLPAWIVNCIVNGTSDERKNEK
SWRAVELNSQGEDVDDFEYPMEPMYKFALPTMRKVMRNFSSQAILMYQNSVAAGKAFVI
KAARNAGYTSIENKWLGIDFLAEAQLSQSQLDIKHQILAANVGRSKTRLFALAAPGDDG
NVDKERHTTHDVSANRHSYSGAAIE"
mat_peptide 1317..2402
/product="P1"
mat_peptide 2403..3284
/product="P3"
mat_peptide 3285..3440
/product="6K1"
mat_peptide 3441..5324
/product="CI"
mat_peptide 5325..5480
/product="6K2"
mat_peptide 5481..6035
/product="VPg"
mat_peptide 6036..6737
/product="NIa-Pro"
mat_peptide 6738..8243
/product="NIb"
mat_peptide 8244..8921
/product="Ham 1-like protein"
mat_peptide 8922..10022
/product="coat protein"
terminator 10288..10540
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 10578..10601
/label=RB
/note="RB"
regulatory complement(10666..10706)
/regulatory_class="terminator"
misc_feature 10716..10739
/label=assembly linker
/note="assembly linker"
CDS complement(10753..11565)
/label=KanR
/note="aminoglycoside phosphotransferase"
misc_feature 11667..11690
/label=assembly linker
/note="assembly linker"
CDS complement(11694..12353)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(12354..13125)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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