LOCUS       40924_29436       13212 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-UCBSV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 13212)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13212)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /note="artificial; tested only in sense orientation"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        514..1183
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1183..10275
                     /note="DSMZ: PV-0912; Ugandan cassava brown streak virus
                     isolate Ke_125"
     CDS             1317..10025
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="ASG92166.1"
                     /translation="MSTIQLFKTIRFGSFEPVKLDEGNNIIEKVPVDLLAGNDGSGPEE
                     QSEQKYHRKESGESWRKVTDLYSVIGNSVYCRSYVAMKNFLNDTKWGGLFKNKKGQELK
                     AAARLRRATSYGFMYDPVACAFECPVCRTKATALEAFTSDCDHCFEIKHIDDDRIVQTE
                     TKFYPINPIELDVEDNLVEAASLEWLKGDVEERVVDRVLLLEDKEIHVTKKALVKRRAE
                     TKLVANAADLTKKLTEICCESGIPIIDIDNSKRKAIPMVKLKHIFGKVECDDMFEEDRY
                     FLEHSNAGKIFRSCEKITYRMIRPGWSGAIILRDNVQSEDHDKFDFINDICVVQGKNLI
                     SYKIENAMRVKTETELDLIDLYSFNLSWAKSKDKFIKHFESDTTQLVRSCCTPSTLWLY
                     ARKARFYKFVDHMILKGSPIIDILVKMEYVGKHLDMFNSVEDVCTEYSHFMKELISETV
                     NDKSDPDVLRVRNLIRAHFESVLEYNKYELIDRIIEKKTQLEAQEIMSRELIKHQYGEL
                     FSWRERLCLKLGIGSSNLYTYWVEREEKQSEKSNVISCIISKPGIEMLINWVSEVCKSK
                     YHSLVNCVDSGILFLWSRIVHLARVSVYGYWNLWFRQAMCVLFIFLVSNFSGKIVSYLK
                     KLIVSEKKLAIKNEEGFVEVQGRKEESFVLKWCAAATLFLSFLNYDWAVGCVSAIGKMK
                     TIFSALGPDFIEKQDGDDDLKFTTFEVEIPGDSRSSSAQTFGEWIEHCIKFNLVSIEPT
                     TSGPMLTLERGKANELADQINCLNATDIRVHGGVGTGKSTALPYELIRYGAVLVCVPTR
                     VLANALHESFMSLFGFDVSLAYRGRVRTGSKPITIMTYGYALNHFHHNPRNLAQFQFII
                     MDEVHTFPVHLNPLFSLLRELSPDKKIIKTSATHVGHSVDLSTNHKVDIHTLEIMDVKK
                     WAELQGTSVFGDVTKEPGNVLVFVASYRDVDVCSDKLKDKGFPVIKVDGRNFRKNTEVQ
                     KMVDGMQGEVKFIVATNIIENGVTLDVDVVVDFGERISPNLCSEDRCILMQRQRISQAE
                     RKQRFGRVGRMKRGSVYKFGRETLPDSMRNRVGSTESALICFAYGLKPVVDDVDIGSVR
                     SVTQRQALTASMFEANYIFTAHLVDKQGFMPRPVFELMKSLLLHTDAVGVSSTYLATNM
                     SGWRRLKEYIRIDDNSRHVQEVQIPWYCSDMSDDFIVKLTECVKAAKPKSQCGYKVDNV
                     DFHTVAHKISVGESNIDESRALVATILDEVKQWRDGITYHSSTPRNKSLMSLMVGWIPR
                     KAEKTKEILDNRIQRLELLLNQLNGVRGIDDYESLVRFFSENPHSAEYLESQCASDYIE
                     EKVMSVKRNYDKSLILGMVGLAVATGTFAYWYMRRSAAVELVEKQAKHKYNRDKRTGRL
                     MFDMDDRETYENFGPEYTDDVISAKMTKAQKERDSKKKGWKAGKINRPMRVFHQLYGVN
                     PLEFDEVVMRVGKLETEPWDVKELNVDAMMIELDDDYHILRDDRMFGKKVSLAFKKEGA
                     DEETIVNLTPHRSKMTSSMSLAPMGFPEEEGRWRQTGAPLIRKIEKEDEVEVQVAKPES
                     TNPYDHILVRLGRAHLGTRVLNCFFHGSKCVIPYHLAEKGDREESLVIATTRGQFDFGP
                     MKNIKCRKVTDYDITICPLPNDVQPFRSKIVMREPKLGEEVVIVCFTRINGKIVMKVSD
                     KSTTYPAGGQFAHLWAYKYDGQPGDCGGPIVATVDQKVVGFHSGVIRNSREEKLRAVYT
                     PVNQELLNCISGDIQMTDFWTFNPDLVEWNSVARVSTFFPMTKAINTITVQANEGEELI
                     DGNLMIVGYVNREVYHNHVIKGKRESFMRYCEQFPNCAFTKELRDQYLPSILSKPAFRK
                     GLLKYNEPVRVGSVNFSCLIRAYLKVEEMFEDLGFLEEAGPQWDPIEILDDLNKKAAMG
                     ALYQGKKQDWLKSIEPADFITAVRESFKHLAGGDVGIWSGSLKAELRPVEKVLEQKTRV
                     FTGAPIDLLLGGKILVDNFNHFFYFNHLKGPWTVGINKFNKGWDRLARYFNHSWNFIDC
                     DGSRFDTSLAPILFQLVCHMREKFGNFDDIERAALRNLYTQIVYTPILTIDGYITKKHR
                     GNNSGQPSTVVDNTIILMIVVEYCKAVMESEGRVMQFKYMCNGDDLILNVPDDEVSIVQ
                     SRFRELFSECGLDYNFDDVHKSIETIEYMSHSFMLKDGVYIPKLKKERIVAILEWERGD
                     EIMRTRSALNAAYIESYGYDDLMVEIERYAVFWATDKGCEYPLLDRKRVEGLYKDDYTD
                     INEEWLIGILPPSFEHCYVDTQTKDLRGREKLELRIESHDRTLQMQMKFPVTFVTGNLG
                     KLAEVKSILGIANDVMAKNIDLPEVQGTPEEIVRKKAQLAVKMTNSPVLVEDTCLCFNA
                     FNGLPGPYIKWFLKELGLDGVVKMLSAFGDKSAYALCTFAYVHNESSDPIVFKGVVNGE
                     IVPPRGNNGFGWDPIFKPDGCGCTFAEMPSSIKNDFSHRRRALEKVKLFLDNLMVKQEK
                     NKASVALTIDVQALNQEEIEAEITALKKLWKDNGPTRTRSSFEARRLRAPQVERVNELL
                     QKLKDEGLQTKKRPCGEPDDGEVVDDDSNDGDNQRSEKEVVDESQNNQQVDPRKLKFKI
                     RGDGNAIRRDDIDKIPTNALEIKKTFKPPKVSQSAYIWIPRSQRDNLTPDVIQNFLAYI
                     PPSHAIDNQLASGIEVENWAIEVAKAYGVNIQEFYRTVLPAWIVNCIVNGTSDERKNEK
                     SWRAVELNSQGEDVDDFEYPMEPMYKFALPTMRKVMRNFSSQAILMYQNSVAAGKAFVI
                     KAARNAGYTSIENKWLGIDFLAEAQLSQSQLDIKHQILAANVGRSKTRLFALAAPGDDG
                     NVDKERHTTHDVSANRHSYSGAAIE"
     mat_peptide     1317..2402
                     /product="P1"
     mat_peptide     2403..3284
                     /product="P3"
     mat_peptide     3285..3440
                     /product="6K1"
     mat_peptide     3441..5324
                     /product="CI"
     mat_peptide     5325..5480
                     /product="6K2"
     mat_peptide     5481..6035
                     /product="VPg"
     mat_peptide     6036..6737
                     /product="NIa-Pro"
     mat_peptide     6738..8243
                     /product="NIb"
     mat_peptide     8244..8921
                     /product="Ham 1-like protein"
     mat_peptide     8922..10022
                     /product="coat protein"
     terminator      10288..10540
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10578..10601
                     /label=RB
                     /note="RB"
     regulatory      complement(10666..10706)
                     /regulatory_class="terminator"
     misc_feature    10716..10739
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(10753..11565)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    11667..11690
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(11694..12353)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(12354..13125)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtcc
      241 agtacgcacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga ttggagtctc
      301 taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa tagaggacct
      361 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
      421 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
      481 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
      541 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
      601 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
      661 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      721 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca
      781 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
      841 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
      901 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      961 cgataaagga aaggccatcg ttgaagatcc tctgccgaca gtggtcccaa agatggaccc
     1021 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     1081 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     1141 gacccttcct ctatataagg aagttcattt catttggaga ggaaaaataa atatgacata
     1201 agaatacata aagatacatc gcatacaaac actgtgcatt caaattcgtg agaaagcaag
     1261 ctgttacgaa agtttacgat atattcttat atattcatag cactgaatac ctgcagatgt
     1321 cgactatcca gctattcaag actatcagat ttggttcctt tgagcctgtt aagctcgatg
     1381 aaggaaacaa tattatagag aaggtcccag ttgaccttct ggcaggaaat gatggctctg
     1441 gtcctgagga gcaatcagaa cagaaatacc ataggaaaga aagtggtgaa agttggcgga
     1501 aagtaaccga cttatattct gtaattggaa attctgttta ctgccgcagt tacgtagcaa
     1561 tgaagaactt cctcaatgac acaaagtggg gaggtctttt taaaaataag aaggggcaag
     1621 aattaaaggc tgcagcaagg ctcagaaggg ctacaagcta tggattcatg tatgatccag
     1681 ttgcttgtgc ctttgaatgt cctgtttgca ggacgaaagc aacagcattg gaagcattca
     1741 caagcgattg tgatcattgt tttgaaatca agcacattga cgatgacagg atcgttcaga
     1801 ctgaaacaaa gttttatcca ataaatccta ttgaactgga tgttgaggat aacttagtgg
     1861 aagctgcatc gcttgaatgg ttgaaaggtg atgtggaaga gagagttgtg gatagggtct
     1921 tactacttga ggataaggaa atccacgtca cgaagaaagc cctagtcaaa agaagagctg
     1981 agacaaaact cgtggcaaat gctgctgatc ttaccaagaa attgactgag atctgttgtg
     2041 agtctggaat tcctatcatt gatattgata atagtaagag gaaagccatt ccaatggtca
     2101 aactcaagca tatctttggg aaagttgagt gtgatgacat gtttgaagaa gatcggtatt
     2161 tcctggagca cagcaatgca gggaaaattt tccgatcatg cgagaaaatt acttatagga
     2221 tgattcgtcc tggatggagt ggagcaatca ttttaagaga taatgttcag agtgaggatc
     2281 atgataagtt tgattttatc aatgatattt gtgttgttca agggaagaat ctgattagtt
     2341 acaaaattga gaatgccatg cgcgttaaga ctgaaacaga attggattta attgatttgt
     2401 attccttcaa tctcagttgg gcgaaatcga aagataagtt cattaagcat tttgaaagtg
     2461 atacaactca attagttaga tcatgttgta ctcctagtac attgtggttg tatgcacgga
     2521 aagccaggtt ttacaaattt gtggatcaca tgatcttaaa gggtagtcca ataattgata
     2581 ttctagttaa aatggagtat gtaggcaagc atctggacat gttcaatagt gttgaagatg
     2641 tgtgtactga gtattctcat tttatgaagg agttgattag cgaaactgtt aatgacaagt
     2701 cagatccaga tgtgttgcgt gttagaaatc tgattagagc gcattttgag tcagttttag
     2761 aatataataa gtatgagttg atagatagga ttattgagaa aaaaacacag ttagaagcac
     2821 aagaaattat gtctcgcgag ctcatcaaac atcagtacgg agagttattt tcttggcgcg
     2881 aacgtttgtg tttgaaacta ggtataggtt catcaaatct ctatacgtac tgggtggaac
     2941 gagaggaaaa acaatcagag aaatcgaatg taatctcttg catcatttcc aaaccgggaa
     3001 tagagatgtt gatcaattgg gtctcagaag tgtgtaagag taaatatcat agtcttgtga
     3061 attgtgtcga tagtggtatt ttattcttgt ggtctagaat agttcattta gcgagagttt
     3121 cagtttatgg ttattggaat ctttggttta gacaagcaat gtgtgtgttg ttcattttct
     3181 tagtttcaaa tttctctggt aaaatagtta gttatcttaa gaaattaatt gtgagtgaga
     3241 agaagctagc tataaagaat gaagaaggtt ttgttgaagt gcaaggtcga aaggaagagt
     3301 cttttgttct aaagtggtgt gctgctgcaa ctttgtttct tagtttcctt aactatgact
     3361 gggcagttgg gtgtgtttca gctattggaa agatgaaaac aatatttagc gcactaggtc
     3421 cagattttat agaaaagcag gatggggatg atgatcttaa attcacaaca tttgaagttg
     3481 agattcctgg agattctaga agttcaagtg cacaaacgtt tggggaatgg attgagcatt
     3541 gcattaaatt caacttagtt tcaattgaac caaccacgag tggacccatg ctaactttgg
     3601 agcgtgggaa agcgaatgag ttggctgacc agataaattg tttgaatgca acagacatac
     3661 gtgtgcatgg tggtgttgga acgggtaagt caacagctct accatatgag ctaataaggt
     3721 atggggcagt tcttgtgtgt gtgcctacga gagttctggc aaatgcattg catgagtcat
     3781 tcatgagctt gtttggattt gatgtgtcat tggcttatcg gggtcgagtc agaactggtt
     3841 caaaaccaat tactatcatg acatatggat atgccttgaa tcatttccat cacaatccca
     3901 ggaatttagc acagtttcaa ttcataataa tggatgaggt tcacacattc cctgttcatt
     3961 taaatccttt attctcattg ttgcgtgagt tgagtcctga caagaaaata atcaagacat
     4021 cagcaacaca tgttggccat agtgttgatc tatcaactaa ccacaaagtt gatatacaca
     4081 cgcttgagat aatggatgtc aagaaatggg ctgaattaca aggaacatct gtgtttggtg
     4141 atgtgacaaa ggaacctgga aatgttttgg tgtttgttgc atcatatagg gatgttgatg
     4201 tttgttcaga taagcttaag gataaaggat ttcctgtaat taaagttgat ggaagaaatt
     4261 tcaggaagaa tacagaagtt cagaaaatgg tggacggaat gcaaggagag gtgaagttca
     4321 ttgtcgcgac taatatcatc gaaaatggtg ttacccttga cgttgatgta gtcgttgatt
     4381 ttggcgaacg cataagtcca aatttatgct cagaagatag gtgcatttta atgcagaggc
     4441 aaagaatctc acaagctgaa agaaaacaaa gatttgggag agttgggaga atgaagagag
     4501 gctcagtgta caaatttgga agggagacgt tgcctgattc aatgagaaat agagtggggt
     4561 caacagagag tgcattaatt tgttttgctt acggtttaaa accagtagtt gatgatgtgg
     4621 atatagggtc agttaggagt gtcacacaga ggcaagcttt gacagcatct atgtttgaag
     4681 cgaattacat attcacagct cacttagtgg acaaacaagg tttcatgcct aggccggttt
     4741 ttgagttgat gaaaagtctt ctgctacata cagatgcggt tggggtcagt agtacatatt
     4801 tagctactaa tatgagtggt tggagaaggt tgaaagagta tattagaatt gacgataact
     4861 cacgtcacgt gcaagaagtt caaattccat ggtactgttc agacatgagt gacgatttta
     4921 ttgttaagtt aactgagtgt gttaaagctg caaagccaaa atcacagtgt ggttacaaag
     4981 tagataatgt agattttcat accgtagctc ataaaataag tgtaggagag tcgaatatag
     5041 atgagtcaag ggctttagtg gcaacaattt tggatgaagt caaacagtgg agagatggta
     5101 ttacatacca ttcaagcaca cctaggaata agagtctgat gagtttgatg gttggatgga
     5161 ttccaaggaa agcagagaaa acaaaggaaa ttcttgacaa tcggattcag cgtctcgagt
     5221 tactcttgaa ccaattaaat ggagtcagag gaatcgatga ctatgaatct cttgttcggt
     5281 ttttcagtga gaatcctcat tcagcggaat atcttgaatc acaatgtgct agtgattata
     5341 tcgaagagaa ggttatgagt gtcaaaagaa actacgataa gtcgctaatc cttggtatgg
     5401 ttggtttagc tgttgcaaca ggcacgtttg cttactggta tatgaggaga agtgctgcgg
     5461 ttgaactcgt tgaaaaacaa gcgaagcaca agtataacag agataagcga acaggaagat
     5521 tgatgtttga tatggatgat agagagactt atgagaattt tggtcctgaa tacacagatg
     5581 atgtcatatc tgccaagatg acaaaagctc agaaagaaag agactcaaag aagaagggat
     5641 ggaaagcagg taaaataaat cgacctatga gggtgtttca tcaattgtat ggagttaatc
     5701 ctcttgagtt cgatgaagtt gtcatgagag ttggtaaact tgagactgaa ccgtgggatg
     5761 tgaaagaatt aaatgttgat gcaatgatga tcgaattgga tgatgattac cacattttac
     5821 gggatgatcg aatgtttggg aagaaagttt ctttagcttt caagaaagaa ggggcagatg
     5881 aggaaaccat tgtgaatttg actccacacc gatcaaaaat gacaagtagc atgagcttag
     5941 ccccaatggg atttccagaa gaagagggta gatggaggca gacaggagca ccgctgataa
     6001 ggaagatcga aaaggaagat gaagttgaag ttcaagttgc taagccagag tcaacaaatc
     6061 cttacgatca tatcttggtt agacttggca gagcgcatct tgggactcgt gtattgaatt
     6121 gtttctttca tgggtcaaaa tgtgttattc catatcattt agctgagaaa ggagataggg
     6181 aagaatcatt ggttattgca accacgagag gacagtttga ttttggacca atgaaaaata
     6241 tcaagtgcag gaaggtcaca gactatgata tcactatttg cccactgcca aatgatgttc
     6301 aaccattccg ttcaaaaatt gtaatgcgtg aaccaaagct aggagaggaa gtggttattg
     6361 tgtgctttac caggatcaat gggaagatcg tgatgaaagt tagtgataag agcaccacat
     6421 atcccgctgg tgggcagttt gcgcaccttt gggcgtataa atacgatggc caaccaggag
     6481 attgcggagg cccaattgtt gcgacagttg atcaaaaggt ggtggggttc catagtggtg
     6541 tgattagaaa tagtagagag gagaaactgc gagctgtgta cactccagta aatcaagagc
     6601 ttttgaattg tatcagtggc gatatccaaa tgacggattt ttggacattc aatcctgatc
     6661 ttgttgagtg gaattccgta gcaagagtgt caacattttt tcctatgaca aaagcgatta
     6721 ataccatcac ggtgcaagcg aatgaaggtg aagaattaat tgatggtaac ttaatgattg
     6781 ttggttatgt taaccgcgag gtttatcata atcacgtcat taaagggaaa agagagagtt
     6841 tcatgagata ttgtgaacaa ttcccaaatt gtgcttttac caaggaattg cgagaccaat
     6901 atcttccaag cattttgagt aagccagctt ttaggaaagg attgttgaag tacaatgagc
     6961 cagttcgagt tgggtcagtg aatttctcgt gcttgatacg agcttatttg aaagtggaag
     7021 aaatgtttga ggatctagga tttctggagg aagccggacc acagtgggac cctattgaaa
     7081 ttttggatga tttgaataag aaagccgcaa tgggcgcatt gtatcaagga aagaagcaag
     7141 actggttgaa gtccatagag cccgcagatt ttatcacggc agtgcgtgaa agtttcaaac
     7201 acttggctgg tggggatgtt ggaatttgga gtggctcact caaagcagaa ttgagacctg
     7261 ttgagaaagt gcttgagcag aaaacaagag tgtttactgg agctccaatt gatttgctgt
     7321 tggggggaaa gattttagtt gataatttta atcatttctt ttattttaat catttgaagg
     7381 ggccttggac tgtaggaatt aataaattta ataaaggttg ggatagatta gctaggtact
     7441 ttaatcatag ttggaacttc atagattgtg atggtagtag gtttgatact tctttagctc
     7501 ccattttgtt tcaattagtg tgccatatgc gagaaaaatt tggaaatttt gatgatattg
     7561 agagggcagc tcttcgtaat ttatacacac agatagtgta tacgccaatt ttaacaattg
     7621 acggatacat tactaagaag catcgtggaa ataatagtgg acaaccttct acagttgtcg
     7681 acaacactat tatactaatg attgttgtgg aatattgcaa ggctgtcatg gagagtgaag
     7741 gaagagtaat gcaattcaag tatatgtgta atggggatga tctgatcctc aatgttcctg
     7801 atgatgaggt gagcatagtt cagagtaggt ttagagagtt gttttcagaa tgtggtttag
     7861 attataattt tgatgatgtt cataagtcaa tagaaacaat tgagtatatg agccattcat
     7921 tcatgctgaa agatggtgtg tatattccaa aattgaagaa agaaagaatc gttgcaattc
     7981 ttgaatggga gagaggtgat gagatcatgc gaacgcggag tgctcttaat gctgcttata
     8041 ttgagagtta tggatatgac gatctgatgg ttgaaattga gcggtatgca gttttctggg
     8101 ctaccgataa aggttgtgag tatccattac tggatagaaa gcgtgtagaa ggactttaca
     8161 aagatgatta cacagatatc aatgaagaat ggttgatagg tattttacca ccatcattcg
     8221 aacattgtta tgttgacacg caaactaagg atttgagagg aagagagaag cttgagctga
     8281 gaattgagag tcatgacaga acactccaaa tgcaaatgaa gttcccagtt acatttgtga
     8341 caggaaattt gggaaaatta gcagaggtga agtctattct tggcattgca aatgatgtta
     8401 tggccaagaa cattgattta ccagaagtgc aaggaactcc agaagaaatt gtgagaaaga
     8461 aggctcaatt agcagtgaag atgactaata gtcctgtttt agtcgaagat acatgtctct
     8521 gttttaatgc tttcaatgga cttcccggac catacatcaa atggtttcta aaagaattgg
     8581 gtcttgatgg tgttgtcaaa atgctgtcag catttggaga taaatcagcg tacgcactat
     8641 gtacattcgc atatgtgcac aatgagtcat ctgatccaat tgtgttcaaa ggagttgtga
     8701 atggtgaaat tgtgccacca cgaggtaata atggctttgg ctgggatcct atctttaagc
     8761 ctgatggatg tggttgtacg ttcgcggaaa tgccaagcag cattaagaat gatttttctc
     8821 acagaagaag agctttagag aaagtcaaat tgtttcttga taacttgatg gtgaagcaag
     8881 agaagaataa agcaagtgtg gctctaacga ttgacgttca ggccttaaat caggaggaaa
     8941 tagaagcaga gatcactgct ttaaagaagc tgtggaaaga caatgggcca acaagaacgc
     9001 gtagttcatt tgaggctagg aggttgagag ccccacaagt tgagcgtgta aatgagttac
     9061 ttcagaaact gaaagatgaa ggattgcaaa caaagaagag gccatgtgga gaaccagatg
     9121 atggggaagt ggtagatgat gacagcaatg atggtgacaa tcagagatct gaaaaggagg
     9181 ttgttgacga aagccagaat aatcaacaag ttgatccaag aaagttgaaa ttcaaaatta
     9241 ggggagatgg aaatgctatt agacgggatg acattgataa aattccaact aatgctcttg
     9301 agatcaagaa gacgttcaag cctccaaagg tatcacagtc agcttacatt tggattccac
     9361 gctctcaaag ggataatcta acccctgatg ttattcagaa tttcctggca tatatacctc
     9421 catctcatgc tatagataac caacttgctt caggaattga agttgaaaat tgggcaattg
     9481 aggttgctaa agcatatgga gttaatatac aggaatttta tcgcacagtt ttgccagctt
     9541 ggatagtgaa ctgtatagtg aacggcacta gcgatgaaag gaaaaatgag aagtcatggc
     9601 gagcagtcga gctgaattct cagggagagg atgttgatga ttttgagtat ccaatggaac
     9661 caatgtacaa atttgcattg ccaactatga ggaaggttat gagaaacttt tctagccaag
     9721 ctattctcat gtaccagaat agtgtcgctg caggaaaggc gtttgtgata aaagctgcta
     9781 gaaatgctgg atacacaagt attgaaaaca aatggctagg tatagacttt ctcgccgaag
     9841 ctcaattgtc tcaaagtcaa cttgatatca agcatcagat tctggcagca aatgttggta
     9901 ggtcaaagac aaggctgttt gcattagcag ctcctgggga tgatggcaat gtagataaag
     9961 aaaggcacac aacacacgat gtcagtgcaa acaggcacag ttatagtggt gccgcaattg
    10021 aataaataaa atattagtgt ttctaattag gtttaagtga gattatagct tagttggaaa
    10081 gctaagctat atttcaaatt tgaatttaag tacttaaata ttgtatttta ttttcaagct
    10141 tggtggagtt ttggatagcc aattatattt tggttgggta agccaaaatg tattttggag
    10201 atctttctcc atatcctttt gtttggtgta gaaatacacc ttataaaagt acaaaaaaaa
    10261 aaaaaaaaaa aaaaatgcat gcctgcagat cgttcaaaca tttggcaata aagtttctta
    10321 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    10381 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    10441 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    10501 gataaattat cgcgcgcggt gtcatctatg ttactagatc tctagcctgc aggaattgtt
    10561 gattttgtga tgactgatgg caggatatat gcggttgtaa ttcattttta ttgtctaaat
    10621 ttctgtattt gtttgtttgt tcggttgtaa atttttttgg aagaacaaga aaagaaaaaa
    10681 cacccgttag ggtgttttta gttagtgtgg cgcgccgact tgcgacatgc ggtcctttgc
    10741 aatcaactat tagaaaaatt catccagcat cagatgaaat tgcagtttgt tcatatccgg
    10801 attatcaatg ccatatttct gaaacagacg tttttgcagg ctcgggctaa attcgcccag
    10861 gcagttccac agaatggcca gatcctgata acgatccgca atgcccacac ggcccacatc
    10921 aatgcagcca atcagtttgc cttcatcgaa aatcaggtta tccaggctaa aatcgccgtg
    10981 ggtcaccacg ctatccgggc taaacggcag cagtttatgc atttctttcc acacctgttc
    11041 caccggccag ccgttacgtt catcatcaaa atcgctcgca tccaccaggc cgttgttcat
    11101 acggctctgc gcctgggcca gacgaaacac acgatcgctg ttaaacgggc agttgcacac
    11161 cggaatgcta tgcagacgac gcagaaacac ggccagcgca tccacaatgt tttcgccgct
    11221 atccggatat tcttccagca cctgaaacgc ggttttgccc ggaatcgcgg tggtcagcag
    11281 ccacgcatca tccggggtgc gaataaaatg tttaatggtc ggcagcggca taaattcggt
    11341 cagccagttc agacgcacca tttcatcggt cacatcgttc gccacgctgc ctttgccatg
    11401 tttcagaaac agttccggcg catccggttt gccatacaga cgataaatgg tcgcgccgct
    11461 ctgacccacg ttatcacgcg cccatttata gccatacaga tccgcatcca tgttgctgtt
    11521 cagacgcgga cggctacagc tcgtttcacg ctgaatatgg ctcataacac cccttgtatt
    11581 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
    11641 gtaacatcag agattttgag acacaagatc ggattggcgg ttatgcggtt ctaccggcgc
    11701 ggcagcgtta cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
    11761 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
    11821 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
    11881 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
    11941 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
    12001 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg
    12061 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
    12121 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
    12181 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
    12241 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
    12301 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
    12361 tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg
    12421 gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa
    12481 cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt
    12541 aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa
    12601 gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag
    12661 tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct
    12721 gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc
    12781 gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
    12841 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
    12901 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
    12961 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
    13021 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
    13081 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
    13141 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
    13201 gcccgtaggg gg
//
