pLX-TCTV vector (V004878)

MSDS

Basic Vector Information

Vector Name:: pLX-TCTV

Antibiotic Resistance:: Kanamycin

Length:: 7625 bp

Type:: Binary vector

Replication origin:: pBBR1 oriV

Host:: Plants

Source/Author:: Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, Chen YR.

Promoter:: CaMV 35S (enhanced)

pLX-TCTV vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLX-TCTV vector Sequence

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Download GeneBank File(.gb)

LOCUS       40924_29426        7625 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-TCTV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 7625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol. 
            Methods (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2018) Agricultural Biotechnology Research Center, Academia 
            Sinica, 128 Academia Road, Section 2, Nangang District, Taipei, 
            Taipei 11529, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1203
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1204..4671
                     /note="Turnip curly top virus isolate IR:Zaf:B11:06
                     (1.16x); GU456685"
     CDS             1578..2693
                     /codon_start=1
                     /product="Rep"
                     /label=Rep
                     /protein_id="AYC81460.1"
                     /translation="MPRTPRQYRIHAKNIFLTYPHCHLTKEDALLQLQTIQCPSTKKFI
                     RICRERHDNGEPHLHVLIQFEGKIQLYNPRHFDLRDGGSGRICHPNIQGAKSSSDVKSY
                     IEKDGDYIDWGEFQIDARSARGGQQTANDACAEALNSGTAEAALAVIREKLPKDYIFQF
                     HNLKPNLAAIFNPPPVGYVPKYNHTQFVLTDDILDWLESNFFLEESNSPAGPSKYKVIP
                     SIDRPKSIIIEGPSRTGKTLWARSLGSHNYITGHLDFSTKVYNDDVSYNVIDDVDPHYL
                     KMKHWKHLIGAQKEWQTNLKYGKPRIIKGGIPSIILCNPGDGASYQNFLDKPENEALKS
                     WSLQNSVFTTIEEPLFNITSDQEVEDSTPTV"
     CDS             1738..1995
                     /codon_start=1
                     /product="C4"
                     /label=C4
                     /protein_id="AYC81461.1"
                     /translation="MGNLISMCLSNSKEKSSSITPVISIYEMEVPVASATQIFRELNRA
                     PTSSPISRRTEITSTGVNFRSMHDLHEEVNRRLMMHVQRR"
     CDS             2581..3006
                     /codon_start=1
                     /product="C2"
                     /label=C2
                     /protein_id="AYC81462.1"
                     /translation="MKPLSPGVYKIQSSQQSRNLSSISRVIKKSKTVLLPCKCKFTIHH
                     ECGEGFTHRGTHNSSSINDWNIHTSSTKPVSSQTNIMGPPGERVLQHQITTPIQLQPTE
                     TTGITHGLDRIHGIGSSPEFDWTSIYDDFFEEITLLP"
     CDS             2714..3136
                     /codon_start=1
                     /product="C3"
                     /label=C3
                     /protein_id="AYC81463.1"
                     /translation="MSVVRDLRTGEPITHRQSTIGTYIHQVPNPFHLKLISWDHQENGF
                     FNIRLQLRFNYNLRKQLGLHMAWIEFTVLGRHRSLTGHRFTTIFLKRLLFYLNNLGIIS
                     LNLVINGISHVLFKDFKFVESVINQEYRVAMKEEFY"
     CDS             complement(3133..3963)
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81464.1"
                     /translation="MSGTWSLKRPRWHSPGYDLAPKTPVKRPAVRRALFNNQAQFSRVA
                     ARRRWSKFPVRGNKPYRYRKLKASDYQIYKDKIGGDNGWTVTFSGDCTMLNNYVRGIGR
                     DQRDSVMTKTAHMHFNGVLMANDAFWEAPNYMTMYSWIILDNDPGGTFPKPSDIFDMEY
                     KEFPSMYEVAESVKSRFIVKRKTEHYLRSTGVAFGEKQNYKAPNVGPVKKPIRMKFRNI
                     WQPSEWKDTAGGKYEDLKKGALLYVCICDNKATQFSFNLKGQWTMYFINRDLMY"
     CDS             complement(3818..4195)
                     /codon_start=1
                     /product="V2"
                     /label=V2
                     /protein_id="AYC81465.1"
                     /translation="MIWRIAKDDKRVRAFRYKKRRFYSLSEYLLAPLATHLRLARILPE
                     KCWSVVPAYTDEAYSFLFMLKEETRTPFQILKDEWNVEFKEAEVALSRLRLSTEDTGEE
                     TGGEESPVQQSSPVQSSSCET"
     terminator      4674..4926
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4991..5014
                     /label=RB
                     /note="RB"
     regulatory      5079..5119
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(5166..5978)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(6107..6766)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(6767..7538)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"

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