LOCUS       40924_29426        7625 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLX-TCTV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, 
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the 
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
  PUBMED    30236898
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center, 
            Academia Sinica, 128 Academia Road, Section 2, Nangang District, 
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 7625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol. 
            Methods (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2018) Agricultural Biotechnology Research Center, Academia 
            Sinica, 128 Academia Road, Section 2, Nangang District, Taipei, 
            Taipei 11529, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1203
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1204..4671
                     /note="Turnip curly top virus isolate IR:Zaf:B11:06
                     (1.16x); GU456685"
     CDS             1578..2693
                     /codon_start=1
                     /product="Rep"
                     /label=Rep
                     /protein_id="AYC81460.1"
                     /translation="MPRTPRQYRIHAKNIFLTYPHCHLTKEDALLQLQTIQCPSTKKFI
                     RICRERHDNGEPHLHVLIQFEGKIQLYNPRHFDLRDGGSGRICHPNIQGAKSSSDVKSY
                     IEKDGDYIDWGEFQIDARSARGGQQTANDACAEALNSGTAEAALAVIREKLPKDYIFQF
                     HNLKPNLAAIFNPPPVGYVPKYNHTQFVLTDDILDWLESNFFLEESNSPAGPSKYKVIP
                     SIDRPKSIIIEGPSRTGKTLWARSLGSHNYITGHLDFSTKVYNDDVSYNVIDDVDPHYL
                     KMKHWKHLIGAQKEWQTNLKYGKPRIIKGGIPSIILCNPGDGASYQNFLDKPENEALKS
                     WSLQNSVFTTIEEPLFNITSDQEVEDSTPTV"
     CDS             1738..1995
                     /codon_start=1
                     /product="C4"
                     /label=C4
                     /protein_id="AYC81461.1"
                     /translation="MGNLISMCLSNSKEKSSSITPVISIYEMEVPVASATQIFRELNRA
                     PTSSPISRRTEITSTGVNFRSMHDLHEEVNRRLMMHVQRR"
     CDS             2581..3006
                     /codon_start=1
                     /product="C2"
                     /label=C2
                     /protein_id="AYC81462.1"
                     /translation="MKPLSPGVYKIQSSQQSRNLSSISRVIKKSKTVLLPCKCKFTIHH
                     ECGEGFTHRGTHNSSSINDWNIHTSSTKPVSSQTNIMGPPGERVLQHQITTPIQLQPTE
                     TTGITHGLDRIHGIGSSPEFDWTSIYDDFFEEITLLP"
     CDS             2714..3136
                     /codon_start=1
                     /product="C3"
                     /label=C3
                     /protein_id="AYC81463.1"
                     /translation="MSVVRDLRTGEPITHRQSTIGTYIHQVPNPFHLKLISWDHQENGF
                     FNIRLQLRFNYNLRKQLGLHMAWIEFTVLGRHRSLTGHRFTTIFLKRLLFYLNNLGIIS
                     LNLVINGISHVLFKDFKFVESVINQEYRVAMKEEFY"
     CDS             complement(3133..3963)
                     /codon_start=1
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="AYC81464.1"
                     /translation="MSGTWSLKRPRWHSPGYDLAPKTPVKRPAVRRALFNNQAQFSRVA
                     ARRRWSKFPVRGNKPYRYRKLKASDYQIYKDKIGGDNGWTVTFSGDCTMLNNYVRGIGR
                     DQRDSVMTKTAHMHFNGVLMANDAFWEAPNYMTMYSWIILDNDPGGTFPKPSDIFDMEY
                     KEFPSMYEVAESVKSRFIVKRKTEHYLRSTGVAFGEKQNYKAPNVGPVKKPIRMKFRNI
                     WQPSEWKDTAGGKYEDLKKGALLYVCICDNKATQFSFNLKGQWTMYFINRDLMY"
     CDS             complement(3818..4195)
                     /codon_start=1
                     /product="V2"
                     /label=V2
                     /protein_id="AYC81465.1"
                     /translation="MIWRIAKDDKRVRAFRYKKRRFYSLSEYLLAPLATHLRLARILPE
                     KCWSVVPAYTDEAYSFLFMLKEETRTPFQILKDEWNVEFKEAEVALSRLRLSTEDTGEE
                     TGGEESPVQQSSPVQSSSCET"
     terminator      4674..4926
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4991..5014
                     /label=RB
                     /note="RB"
     regulatory      5079..5119
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(5166..5978)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(6107..6766)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(6767..7538)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcac cagtacgcac gattcaaggc ttgcttcaca aaccaaggca
      301 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
      361 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
      421 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
      481 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      541 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      601 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      661 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      721 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      781 atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
      841 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      901 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      961 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatc ctctgccgac
     1021 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     1081 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     1141 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     1201 aggctccaaa tcatttttta atatacacat ttcctaatac cgatgctaat tatatagccg
     1261 aagcttagct tcggagacaa atagtacaga tacacgtgta atcgcactca ataaccaatc
     1321 tttaatactt tacctttggg accacacgac caagactaat cgtctttact ttcactttgg
     1381 cgcggcttct ccactacgca cctgtcacca tcaaaacacg cgccaaaaat ttttttttgg
     1441 gccatccggt aatattaaac ggatggccca aatgtgagca agaggccttc tacaatccat
     1501 actatatata caaccccagt aaccccgata caatagagtt tgagcgtaac cccaattcat
     1561 ttctacaaat accgaaaatg ccaaggactc cgaggcaata tcgcattcat gctaaaaaca
     1621 ttttcctaac ataccctcac tgccatctaa ctaaagaaga cgctcttctg caactacaaa
     1681 ctatacagtg tccttcaacc aagaaattca tacgaatttg tagagaaagg cacgataatg
     1741 gggaacctca tctccatgtg cttatccaat tcgaaggaaa aatccagctc tataaccccc
     1801 gtcatttcga tttacgagat ggaggttccg gtcgcatctg ccacccaaat attcagggag
     1861 ctaaatcgag ctccgacgtc aagtcctata tcgagaagga cggagattac atcgactggg
     1921 gtgaatttca gatcgatgca cgatctgcac gaggaggtca acagacggct aatgatgcat
     1981 gtgcagaggc gttaaactca ggtaccgctg aagccgcttt ggctgtcatt agggagaaac
     2041 tccctaaaga ttacatcttt caattccata acctgaaacc aaacttagcg gccatattca
     2101 atcctcctcc tgtaggatac gtccccaaat acaaccatac ccaattcgtc ttaacagatg
     2161 atattttgga ttggttagag tctaattttt ttctggagga gtccaattcc cctgcggggc
     2221 cgagtaaata taaggtaatt ccatctatcg acagacctaa atcaataatc atcgaaggtc
     2281 cttcaagaac aggtaaaacc ctgtgggcaa gatcccttgg atctcataat tacataaccg
     2341 gtcatctcga cttctccact aaagtataca acgacgacgt ttcttacaac gtgattgatg
     2401 acgtggaccc ccattatcta aagatgaagc actggaagca tttaattggt gcgcaaaagg
     2461 agtggcagac aaacctcaaa tacgggaagc cacgtattat aaaaggggga atcccttcga
     2521 tcattttatg caacccagga gacggagcct catatcaaaa cttcctcgac aaaccagaga
     2581 atgaagccct taagtcctgg agtttacaaa attcagtctt cacaacaatc gaggaacctc
     2641 tcttcaatat cacgagtgat caagaagtcg aagacagtac tcctaccgtg taagtgcaaa
     2701 ttcacaatac atcatgagtg tggtgaggga tttacgcaca ggggaaccca taactcatcg
     2761 tcaatcaacg attggaacat acatacatca agtaccaaac ccgtttcatc tcaaactaat
     2821 atcatgggac caccaggaga acgggttctt caacatcaga ttacaactcc gattcaacta
     2881 caacctacgg aaacaactgg gattacacat ggcttggatc gaattcacgg tattgggtcg
     2941 tcaccggagt ttgactggac atcgatttac gacgattttt ttgaagagat tactcttcta
     3001 ccttaataat ctagggatta tatcccttaa ccttgtaata aatggtatta gtcatgtttt
     3061 atttaaggat tttaagtttg tcgagtctgt aataaatcaa gagtatcgtg ttgcaatgaa
     3121 agaagagttt tattaataca ttaaatctct atttatgaaa tacatggtcc actgtccctt
     3181 caaattaaac gaaaattgag tagccttgtt atcacaaata catacgtata acaaagcccc
     3241 tttcttcaaa tcctcgtatt ttcctccagc cgtatccttc cactcggaag gctgccatat
     3301 attacgaaac ttcatacgta taggcttttt aaccggacca acattaggcg ccttataatt
     3361 ttgtttctcc ccaaaagcaa caccggtaga ccgtaaataa tgctccgtct ttctcttaac
     3421 aataaaccga gatttaacac tctcggcgac ctcatacatg gacggaaact ctttatactc
     3481 catatcaaat atgtcagagg gctttggaaa tgtaccacca ggatcattat caagaataat
     3541 ccaactatac atcgtcatat aattaggcgc ttcccagaaa gcatcattgg ccatcaatac
     3601 cccattaaaa tgcatatgtg cggtcttagt catcaccgag tcacgctggt ccctaccaat
     3661 tcctcttacg taattattca acatagtgca gtccccacta aatgtcaccg tccacccatt
     3721 atctccacca atcttatcct tgtagatctg ataatcacta gcttttaact tacgatacct
     3781 atacggttta ttacctcgaa caggaaactt cgaccatcta cgtctcgcag ctactcgact
     3841 gaactgggct tgattgttga acagggctct cctcaccgcc ggtctcttca ccggtgtctt
     3901 cggtgctaag tcgtagcctg gagagtgcca cctcggcctc tttaaactcc acgttccact
     3961 catctttcaa tatttgaaac ggcgttcgcg tttcttcttt taacataaac agaaaactat
     4021 acgcctcgtc ggtataagct ggaactacgg accaacactt ctctggtaaa atacgagcga
     4081 gtcgtaaatg tgtagccaaa ggggccaata aatactccga taaagagtaa aaccttcttt
     4141 ttttatacct aaacgcccta acccgtttat cgtcttttgc gattctccaa atcatttttt
     4201 aatatacaca tttcctaata ccgatgctaa ttatatagcc gaagcttagc ttcggagaca
     4261 aatagtacag atacacgtgt aatcgcactc aataaccaat ctttaatact ttacctttgg
     4321 gaccacacga ccaagactaa tcgtctttac tttcactttg gcgcggcttc tccactacgc
     4381 acctgtcacc atcaaaacac gcgccaaaaa tttttttttg ggccatccgg taatattaaa
     4441 cggatggccc aaatgtgagc aagaggcctt ctacaatcca tactatatat acaaccccag
     4501 taaccccgat acaatagagt ttgagcgtaa ccccaattca tttctacaaa taccgaaaat
     4561 gccaaggact ccgaggcaat atcgcattca tgctaaaaac attttcctaa cataccctca
     4621 ctgccatcta actaaagaag acgctcttct gcaactacaa actatacagt gcagatcgtt
     4681 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     4741 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4801 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4861 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     4921 tcgatctcgt caggagacgg gacaaggatg cgagctagcc tgcaggaatt gttgattttg
     4981 tgatgactga tggcaggata tatgcggttg taattcattt ttattgtcta aatttctgta
     5041 tttgtttgtt tgttcggttg taaatttttt tggaagaaca agaaaagaaa aaacacccgt
     5101 tagggtgttt ttagttagtg tggcgcgccg acttgcgaca tgcggtcctt tgcaatcaac
     5161 tattagaaaa attcatccag catcagatga aattgcagtt tgttcatatc cggattatca
     5221 atgccatatt tctgaaacag acgtttttgc aggctcgggc taaattcgcc caggcagttc
     5281 cacagaatgg ccagatcctg ataacgatcc gcaatgccca cacggcccac atcaatgcag
     5341 ccaatcagtt tgccttcatc gaaaatcagg ttatccaggc taaaatcgcc gtgggtcacc
     5401 acgctatccg ggctaaacgg cagcagttta tgcatttctt tccacacctg ttccaccggc
     5461 cagccgttac gttcatcatc aaaatcgctc gcatccacca ggccgttgtt catacggctc
     5521 tgcgcctggg ccagacgaaa cacacgatcg ctgttaaacg ggcagttgca caccggaatg
     5581 ctatgcagac gacgcagaaa cacggccagc gcatccacaa tgttttcgcc gctatccgga
     5641 tattcttcca gcacctgaaa cgcggttttg cccggaatcg cggtggtcag cagccacgca
     5701 tcatccgggg tgcgaataaa atgtttaatg gtcggcagcg gcataaattc ggtcagccag
     5761 ttcagacgca ccatttcatc ggtcacatcg ttcgccacgc tgcctttgcc atgtttcaga
     5821 aacagttccg gcgcatccgg tttgccatac agacgataaa tggtcgcgcc gctctgaccc
     5881 acgttatcac gcgcccattt atagccatac agatccgcat ccatgttgct gttcagacgc
     5941 ggacggctac agctcgtttc acgctgaata tggctcataa caccccttgt attactgttt
     6001 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     6061 cagagatttt gagacacaag atcggattgg cggttatgcg gttctaccgg cgcggcagcg
     6121 ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
     6181 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
     6241 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
     6301 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
     6361 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
     6421 ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac acggtgccgg
     6481 ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt
     6541 ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg accaccacgg
     6601 cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc gtgggtttcc
     6661 tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg
     6721 gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt atctccatgc
     6781 ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt tcggcagcgc
     6841 gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt taacctacgc
     6901 aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt tttaagttgt
     6961 tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc aaagaagggc
     7021 acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa aagtggcccc
     7081 tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc gctgccccct
     7141 tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc ttcgcccttc
     7201 gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg
     7261 cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc
     7321 gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc
     7381 cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca
     7441 atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt
     7501 gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc
     7561 cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta
     7621 ggggg
//
