Basic Vector Information
- Vector Name:
- pLX-B2alpha2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3287 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.
- Promoter:
- lac UV5
pLX-B2alpha2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-B2alpha2 vector Sequence
LOCUS 40924_29346 3287 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pLX-B2alpha2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3287) AUTHORS Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA. TITLE Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins JOURNAL ACS Synth Biol (2017) In press PUBMED 28657330 REFERENCE 2 (bases 1 to 3287) AUTHORS Pasin F, Garcia JA. TITLE Direct Submission JOURNAL Submitted (26-MAR-2017) Department of Plant Molecular Genetics, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3287) TITLE Direct Submission REFERENCE 4 (bases 1 to 3287) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol (2017) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-MAR-2017) Department of Plant Molecular Genetics, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 28049, Spain" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3287 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..24 /label=assembly linker /note="assembly linker" regulatory 25..72 /regulatory_class="terminator" misc_feature 133..157 /label=LB T-DNA repeat /note="left border repeat from octopine Ach5 T-DNA" misc_feature 184..208 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS complement(312..482) /label=lacZ-alpha /note="LacZ-alpha fragment of beta-galactosidase" promoter complement(531..561) /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" misc_feature 653..676 /label=RB /note="RB" regulatory complement(741..781) /regulatory_class="terminator" misc_feature 791..814 /label=assembly linker /note="assembly linker" CDS complement(828..1640) /label=KanR /note="aminoglycoside phosphotransferase" misc_feature 1742..1765 /label=assembly linker /note="assembly linker" CDS complement(1769..2428) /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" rep_origin complement(2429..3200) /direction=LEFT /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
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