LOCUS       40924_29346        3287 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-B2alpha2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3287)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(312..482)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(531..561)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(828..1640)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1742..1765
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1769..2428)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2429..3200)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag tcaggagaga gaccaaaagc aaaaacccgc cgaagcgggt
      301 tactagcgcc attcgccatt cagagagcgg agctgctgcg acggacgatc ggtacgcgcc
      361 tcttcgctat tacgccaact ggcgaaaggt ggatgtgctg caaggcgatt aagttgggta
      421 acgccagggt tttcccagtc acgacgttgt agtaccacgg caaggctatc tgtaatcatt
      481 gttgtactcc ggttaggacg gattgggaac tggctaactc aaaatccaca cattatacga
      541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtctct cgctggagac
      601 gggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgcaatca actattagaa aaattcatcc
      841 agcatcagat gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac
      901 agacgttttt gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc
      961 tgataacgat ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca
     1021 tcgaaaatca ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac
     1081 ggcagcagtt tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca
     1141 tcaaaatcgc tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga
     1201 aacacacgat cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga
     1261 aacacggcca gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga
     1321 aacgcggttt tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata
     1381 aaatgtttaa tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca
     1441 tcggtcacat cgttcgccac gctgcctttg ccatgtttca gaaacagttc cggcgcatcc
     1501 ggtttgccat acagacgata aatggtcgcg ccgctctgac ccacgttatc acgcgcccat
     1561 ttatagccat acagatccgc atccatgttg ctgttcagac gcggacggct acagctcgtt
     1621 tcacgctgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1681 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1741 agatcggatt ggcggttatg cggttctacc ggcgcggcag cgttacccgt gtcggcggct
     1801 ccaacggctc gccatcgtcc agaaaacacg gctcatcggg catcggcagg cgctgctgcc
     1861 cgcgccgttc ccattcctcc gtttcggtca aggctggcag gtctggttcc atgcccggaa
     1921 tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc tcgcccggat
     1981 acagggtcgg gatgcggcgc aggtcgccat gccccaacag cgattcgtcc tggtcgtcgt
     2041 gatcaaccac cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg ggctggcccc
     2101 acgccacgcg gtcattgacc acgtaggccg acacggtgcc ggggccgttg agcttcacga
     2161 cggagatcca gcgctcggcc accaagtcct tgactgcgta ttggaccgtc cgcaaagaac
     2221 gtccgatgag cttggaaagt gtcttctggc tgaccaccac ggcgttctgg tggcccatct
     2281 gcgccacgag gtgatgcagc agcattgccg ccgtgggttt cctcgcaata agcccggccc
     2341 acgcctcatg cgctttgcgt tccgtttgca cccagtgacc gggcttgttc ttggcttgaa
     2401 tgccgatttc tctggactgc gtggccatgc ttatctccat gcggtagggg tgccgcacgg
     2461 ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa ttatgcgttg
     2521 cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct attgcggggg
     2581 gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt aagtctttcg
     2641 catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc ggggttcccc
     2701 cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc ttgttgatcg
     2761 actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc ccgcactcgc
     2821 cgccgtgagg ctcggggggc aggcgggcgg gcttcgccct tcgactgccc ccactcgcat
     2881 aggcttgggt cgttccaggc gcgtcaaggc caagccgctg cgcggtcgct gcgcgagcct
     2941 tgacccgcct tccacttggt gtccaaccgg caagcgaagc gcgcaggccg caggccggag
     3001 gcttttcccc agagaaaatt aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg
     3061 agccggtggg tatgtggtcg aaggctgggt agccggtggg caatccctgt ggtcaagctc
     3121 gtgggcaggc gcagcctgtc catcagcttg tccagcaggg ttgtccacgg gccgagcgaa
     3181 gcgagccagc cggtggccgc tcgcggccat cgtccacata tccacgggct ggcaagggag
     3241 cgcagcgacc gcgcagggcg aagcccggag agcaagcccg taggggg
//
