Basic Vector Information
- Vector Name:
- pLvCmvLacZ
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10423 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, Atsma DE.
- Promoter:
- hPGK
pLvCmvLacZ vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLvCmvLacZ vector Sequence
LOCUS 40924_28931 10423 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pLvCmvLacZ, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10423)
AUTHORS van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
Atsma DE.
TITLE Fibroblasts from human postmyocardial infarction scars acquire
properties of cardiomyocytes after transduction with a recombinant
myocardin gene
JOURNAL FASEB J. 21 (12), 3369-3379 (2007)
PUBMED 17579192
REFERENCE 2 (bases 1 to 10423)
AUTHORS van Tuyn J.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
RC, Netherlands
REFERENCE 3 (bases 1 to 10423)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10423)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10423
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 1799..1916
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2021..2324
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2465..2948
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 3111..6164
/label=lacZ
/note="beta-galactosidase"
misc_feature 6210..6798
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 6937..7170
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7242..7376
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 7403..7538
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(7559..7577)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7587..7603)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 7748..8203
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 8232..8336
/label=AmpR promoter
CDS 8337..9194
/label=AmpR
/note="beta-lactamase"
rep_origin 9368..9956
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 10244..10265
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10281..10311
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10319..10335
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10343..10359
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 10380..10398
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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