pLvCmvLacZ vector (V004906)

LOCUS       V004906                10423 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004906
VERSION     V004906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire
            properties of cardiomyocytes after transduction with a recombinant
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
   PUBMED   17579192
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
            RC, Netherlands
REFERENCE   3  (bases 1 to 10423)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2021..2324
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2465..2948
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3111..6164
                     /label="lacZ"
                     /note="beta-galactosidase"
     misc_feature    6210..6798
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6937..7170
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7242..7376
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7403..7538
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7559..7577)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7587..7603)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7748..8203
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8232..8336
                     /label="AmpR promoter"
     CDS             8337..9194
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9368..9956
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    10244..10265
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10281..10311
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10319..10335
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10343..10359
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10380..10398
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"