LOCUS       V004906                10423 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004906
VERSION     V004906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire
            properties of cardiomyocytes after transduction with a recombinant
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
   PUBMED   17579192
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
            RC, Netherlands
REFERENCE   3  (bases 1 to 10423)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2021..2324
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2465..2948
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3111..6164
                     /label="lacZ"
                     /note="beta-galactosidase"
     misc_feature    6210..6798
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6937..7170
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7242..7376
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7403..7538
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7559..7577)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7587..7603)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7748..8203
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8232..8336
                     /label="AmpR promoter"
     CDS             8337..9194
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9368..9956
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    10244..10265
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10281..10311
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10319..10335
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10343..10359
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10380..10398
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 acctgaggta attataaccc atcgcatgcc tgcagagctc tagttattaa tagtaatcaa
     1981 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2041 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2101 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt
     2161 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2221 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2281 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2341 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2401 ttgacgtcaa tgggagtttg ttttggcgac gggcccggta ccgcggccaa ttcgataatt
     2461 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     2521 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     2581 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     2641 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     2701 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     2761 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     2821 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     2881 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     2941 ccctcgttcc gaattggccg cacttaagtt acgcgtggat ccacgcgtaa cttaagtgcg
     3001 gccgcggtac catggataaa gttttccgga attccgccaa aaagaagaga aaggtagaag
     3061 accccaagga ctttccttca gaattgctaa gttttttgag tccaagcttg gcactggccg
     3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3241 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
     3301 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
     3361 actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg
     3421 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
     3481 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg
     3541 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt
     3601 ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc
     3661 tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt
     3721 tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca
     3781 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg
     3841 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca
     3901 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg
     3961 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct
     4021 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
     4081 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt
     4141 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
     4201 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
     4261 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg
     4321 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
     4381 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
     4441 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
     4501 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
     4561 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
     4621 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag
     4681 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt
     4741 tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg
     4801 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
     4861 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg
     4921 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
     4981 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
     5041 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc
     5101 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg
     5161 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt
     5221 cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga
     5281 cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca
     5341 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt
     5401 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc
     5461 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac
     5521 gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata
     5581 cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct
     5641 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
     5701 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg
     5761 cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
     5821 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt
     5881 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
     5941 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg
     6001 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
     6061 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc
     6121 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataaagg taccgggccc
     6181 gtcgactcta gaggtaccgg ttgttcgata atcaacctct ggattacaaa atttgtgaaa
     6241 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6301 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6361 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6421 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6481 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6541 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6601 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6661 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6721 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6781 tttgggccgc ctccccgcct gatcgaacgt tagccggcta catggcgcaa tcactagagc
     6841 ggccgccacc gcggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     6901 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6961 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7021 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7081 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7141 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7201 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7381 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7561 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7741 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7801 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7861 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7921 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7981 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8041 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8101 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8161 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccaggt ggcacttttc
     8221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9481 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10261 ctcattaggc accccagggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10321 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
    10381 attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
