Basic Vector Information
- Vector Name:
- pLV.pA+.GS.DsRed
- Length:
- 8709 bp
- Type:
- Lentivirus shuttle vector
- Replication origin:
- ori
- Source/Author:
- Goncalves MA, Janssen JM, Holkers M, de Vries AA.
- Promoter:
- RSV
pLV.pA+.GS.DsRed vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV.pA+.GS.DsRed vector Sequence
LOCUS V004908 8709 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004908
VERSION V004908
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8709)
AUTHORS Goncalves MA, Janssen JM, Holkers M, de Vries AA.
TITLE Rapid and sensitive lentivirus vector-based conditional gene
expression assay to monitor and quantify cell fusion activity
JOURNAL PLoS ONE 5 (6), E10954 (2010)
PUBMED 20532169
REFERENCE 2 (bases 1 to 8709)
AUTHORS Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands
REFERENCE 3 (bases 1 to 8709)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8709)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-DEC-2009) Molecular Cell Biology, Leiden University Medical
Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8709
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1799..1916
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind complement(1943..1990)
/label="FRT"
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
regulatory complement(2003..2506)
/label="Homo sapiens GAPDH promoter"
/note="Homo sapiens GAPDH promoter"
/regulatory_class="promoter"
polyA_signal complement(2907..2962)
/label="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(3140..3814)
/label="DsRed-Express"
/note="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
protein_bind complement(3848..3895)
/label="FRT"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
regulatory complement(3947..4332)
/label="murine metallothionein polyadenylation signal"
/note="murine metallothionein polyadenylation signal"
/regulatory_class="polyA_signal_sequence"
CDS 4693..5133
/gene="X"
/label="Protein X"
/note="Protein X from Hepatitis B virus genotype D subtype
ayw (isolate France/Tiollais/1979). Accession#: P03165"
LTR 5225..5458
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5530..5664
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 5691..5826
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(5847..5865)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5875..5891)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6036..6491
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6520..6624
/label="AmpR promoter"
misc_feature 6809..7186
/label="ISS"
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
rep_origin 7655..8243
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8531..8552
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8567..8597
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 8605..8621
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8629..8645
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 8666..8684
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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