LOCUS       V004908                 8709 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004908
VERSION     V004908
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Goncalves MA, Janssen JM, Holkers M, de Vries AA.
  TITLE     Rapid and sensitive lentivirus vector-based conditional gene
            expression assay to monitor and quantify cell fusion activity
  JOURNAL   PLoS ONE 5 (6), E10954 (2010)
   PUBMED   20532169
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 8709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-DEC-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(1943..1990)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(2003..2506)
                     /label="Homo sapiens GAPDH promoter"
                     /note="Homo sapiens GAPDH promoter"
                     /regulatory_class="promoter"
     polyA_signal    complement(2907..2962)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(3140..3814)
                     /label="DsRed-Express"
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     protein_bind    complement(3848..3895)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(3947..4332)
                     /label="murine metallothionein polyadenylation signal"
                     /note="murine metallothionein polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     CDS             4693..5133
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             5225..5458
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5530..5664
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5691..5826
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5847..5865)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5875..5891)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6036..6491
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6520..6624
                     /label="AmpR promoter"
     misc_feature    6809..7186
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      7655..8243
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8531..8552
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8567..8597
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8605..8621
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8629..8645
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8666..8684
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ggggatcctc tagagtcagc ttgaagttcc tatactttct agagaatagg aacttcggaa
     1981 tagtaacttc aatgatctcg agcaggagga gcagagagcg aagcgggagg ctgcgggctc
     2041 aatttataga aaccgggggc gcggcggcag gcggtgactc ggaccccgcc tcccgccagg
     2101 ctcagccagt cccagcccaa ggtcttgagg cctgagctac gtgcgcccgt aaaaccgcta
     2161 gtagccgggc cctactttct ccccgctttt ttttttccgc agccgcctgg ttcaactggg
     2221 cacgcaccga gcgtgtgggc cccgacgtag ggggaaggga ctgagattgg cccgatggga
     2281 ggtgatcggt gctggttccc aggactggac tgtgggcagc aggacactag ggagtcaagg
     2341 acggggaccc ttacacgctt ggatgaaaca ggaggacttt gggaacgact gagatgggga
     2401 attggagccg gagcccagca ccgctcgcgg ctgcacgcgg cctgccccct cccctttctt
     2461 tctttcaaag gctgagaggc gggaaagttg gggaacttct cctcgaagct gatccaattg
     2521 caatgatcat catgacagat ctgcgcgcga tcgatgggat cagcttgggc tgcaggtcga
     2581 gggatcttca taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa
     2641 atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa
     2701 aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga
     2761 cagcaggggg ctgtttcata tactgatgac ctctttatag ccacctttgt tcatggcagc
     2821 cagcatatgg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     2881 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     2941 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     3001 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
     3061 acaccagcca ccaccttttg ataggcagcc tgcacctgag gagtgaattg atccacgcgt
     3121 aacttaagtg cggccgctac aggaacaggt ggtggcggcc ctcggcgcgc tcgtactgct
     3181 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttggagtcc acgtagtagt
     3241 agccgggcag ctgcacgggc ttcttggcca tgtagatgga cttgaactcc accaggtagt
     3301 ggccgccgtc cttcagcttc agggccttgt ggatctcgcc cttcagcacg ccgtcgcggg
     3361 ggtacaggcg ctcggtggag ggctcccagc ccatagtctt cttctgcatt acggggccgt
     3421 cggaggggaa gttcacgccg atgaacttca ccttgtagat gaagcagccg tcctgcaggg
     3481 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
     3541 tgaagccctc ggggaaggac agcttcttgt agtcggggat gtcggcgggg tgcttcacgt
     3601 acaccttgga gccgtactgg aactgggggg acaggatgtc ccaggcgaag ggcagggggc
     3661 cgcccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
     3721 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgcgcacc ttgaagcgca
     3781 tgaactcctt gatgacgtcc tcggaggagg ccatggtggc gactagtgga tcaacgttag
     3841 ccagcttgaa gttcctatac tttctagaga ataggaactt cggaatagta acttcaatga
     3901 tcatcatgac agatctgcgc gcgatcgata aatgtttgac ctgcagagct tatgggatag
     3961 cttggcttta cccaaagaca ttacccctct gtgagggcca gaggcgggag gtaaaggcac
     4021 gagggggtcc aaaaccagat ccccagacta tcaagtcacc ccagtttctt attgccaaac
     4081 cacaccaaga aaaccagagt tagactcaaa caggctttta ttattcacgt actcggtaga
     4141 aaacgggggt ttagtaaaca gggtggaact gtataggaag acgctgggtt ggtccgatac
     4201 tatttacacg tggtggcagc gctgttcgtc acatcaggca cagcacgtgc acttgtccgc
     4261 ggcgcctttg cagacacagc cctgggcaca tttggagcag cccacgggac agcaggagca
     4321 gcagcctaga aaggaggaca agctgtacaa gtaaagcggc cgcgtcgagc cctatgggta
     4381 ccatgcatgt attcaatcta agcaggcttt cactttctcg ccaacttaca aggcctttct
     4441 gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa cggccaggtc tgtgccaagt
     4501 gtttgctgac gcaaccccca ctggctgggg cttggtcatg ggccatcagc gcatgcgtgg
     4561 aaccttttcg gctcctctgc cgatccatac tgcggaactc ctagccgctt gttttgctcg
     4621 cagcaggtct ggagcaaaca ttatcgggac tgataactct gttgtcctat cccgcaaata
     4681 tacatcgttt ccatggctgc taggctgtgc tgccaactgg atcctgcgcg ggacgtcctt
     4741 tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct tctcggggtc gcttgggact
     4801 ctctcgtccc cttctccgtc tgccgttccg accgaccacg gggcgcacct ctctttacgc
     4861 ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca
     4921 cgtcgcatgg agaccaccgt gaacgcccac caaatattgc ccaaggtctt acataagagg
     4981 actcttggac tctcagcaat gtcaacgacc gaccttgagg catacttcaa agactgtttg
     5041 tttaaagact gggaggagtt gggggaggag attaggttaa aggtctttgt actaggaggc
     5101 tgtaggcata aattggtctg cgcaccagca ccatggcgca atcactagag cggggtacct
     5161 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5221 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5461 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5521 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5641 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5761 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5821 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     5881 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5941 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6001 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag
     6061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6481 gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6901 atacactatt ctcagaatga cttggttgag actcaccagt cacagaaaag catcttacgg
     6961 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7021 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7081 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7141 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7201 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7261 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7321 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7381 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7441 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7801 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8341 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8401 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8461 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8521 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8581 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8641 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8701 tgcaagctt
//
