pLV.hPGK.eGFP vector (V004911)

LOCUS       V004911                 6916 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004911
VERSION     V004911
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Histone deacetylases hinder transgene expression from
            integration-defective lentiviral vectors in dividing and
            non-dividing cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2012) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC,
            Netherlands
REFERENCE   3  (bases 1 to 6916)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6916)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2012) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2033..2516
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2544..3260
                     /label="EGFP"
                     /note="enhanced GFP"
     LTR             3430..3663
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3735..3869
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3896..4031
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4052..4070)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4080..4096)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4241..4696
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4725..4829
                     /label="AmpR promoter"
     CDS             4830..5687
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5861..6449
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6737..6758
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6774..6804
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6812..6828
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6836..6852
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6873..6891
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"