LOCUS       V004911                 6916 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004911
VERSION     V004911
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Histone deacetylases hinder transgene expression from
            integration-defective lentiviral vectors in dividing and
            non-dividing cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2012) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC,
            Netherlands
REFERENCE   3  (bases 1 to 6916)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6916)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2012) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2033..2516
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2544..3260
                     /label="EGFP"
                     /note="enhanced GFP"
     LTR             3430..3663
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3735..3869
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3896..4031
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4052..4070)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4080..4096)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4241..4696
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4725..4829
                     /label="AmpR promoter"
     CDS             4830..5687
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5861..6449
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6737..6758
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6774..6804
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6812..6828
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6836..6852
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6873..6891
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 acctgaggta attataaccc gggccctata tatggatcca attgcaatga tcatcatgac
     1981 agatctgcgc gcgatcgatc ttacgcgtgc tagcccaatt cgataattcc acggggttgg
     2041 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2101 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     2161 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     2221 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     2281 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     2341 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     2401 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2461 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttccga
     2521 attgggctcg accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2581 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2641 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2701 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2761 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2821 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2881 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2941 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3001 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3061 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3121 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3181 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3241 gcatggacga gctgtacaag taaagcggcc gcgactctag aggtaccggt tgttaacgtt
     3301 agccggctac atggcgcaat cactagagcg gccgccaccg cggaattaat tcgagctcgg
     3361 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3421 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3481 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3541 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3601 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3661 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3721 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3841 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3961 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4021 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4081 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4141 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4201 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca
     4261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4441 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4501 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4561 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4621 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4681 ttaacgttta caatttccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4741 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4801 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4861 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4921 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4981 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5041 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5101 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5161 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5221 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5281 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5341 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5401 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5461 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5521 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5581 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5641 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5701 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5761 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5821 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5881 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5941 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6001 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6061 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6601 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6661 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6721 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag ggctttacac
     6781 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6841 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     6901 ctggagctgc aagctt
//
