Basic Vector Information
- Vector Name:
- pLV.hMyoD.eGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9522 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Goncalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de Watering MJ, Knaan-Shanzer S, de Vries AA.
- Promoter:
- RSV
pLV.hMyoD.eGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV.hMyoD.eGFP vector Sequence
LOCUS V004912 9522 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004912
VERSION V004912
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9522)
AUTHORS Goncalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de
Watering MJ, Knaan-Shanzer S, de Vries AA.
TITLE Genetic complementation of human muscle cells via directed stem cell
fusion
JOURNAL Mol. Ther. 16 (4), 741-748 (2008)
PUBMED 18334989
REFERENCE 2 (bases 1 to 9522)
AUTHORS Goncalves MA, de Vries AA.
TITLE Direct Submission
JOURNAL Submitted (23-JUL-2007) Molecular Cell Biology, Leiden University
Medical Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands
REFERENCE 3 (bases 1 to 9522)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9522)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Ther."; date: "2008"; volume: "16"; issue: "4"; pages: "741-748"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-JUL-2007) Molecular Cell Biology, Leiden University Medical
Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9522
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1799..1916
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1939..2242
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2243..2446
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2631..3590
/gene="MYOD1"
/label="Myoblast determination protein 1"
/note="Myoblast determination protein 1 from Homo sapiens.
Accession#: P15172"
misc_feature 3864..4424
/label="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4447..5163
/label="EGFP"
/note="enhanced GFP"
CDS 5505..5945
/gene="X"
/label="Protein X"
/note="Protein X from Hepatitis B virus genotype D subtype
ayw (isolate France/Tiollais/1979). Accession#: P03165"
LTR 6037..6270
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6342..6476
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 6503..6638
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(6659..6677)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6687..6703)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6848..7303
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7332..7436
/label="AmpR promoter"
CDS 7437..8294
/label="AmpR"
/note="beta-lactamase"
rep_origin 8468..9056
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9344..9365
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9380..9410
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 9418..9434
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9442..9458
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 9479..9497
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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