pLV.hMyoD.eGFP vector (V004912)

LOCUS       V004912                 9522 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004912
VERSION     V004912
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Goncalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de
            Watering MJ, Knaan-Shanzer S, de Vries AA.
  TITLE     Genetic complementation of human muscle cells via directed stem cell
            fusion
  JOURNAL   Mol. Ther. 16 (4), 741-748 (2008)
   PUBMED   18334989
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Goncalves MA, de Vries AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands
REFERENCE   3  (bases 1 to 9522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Ther."; date: "2008"; volume: "16"; issue: "4"; pages: "741-748"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-JUL-2007) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1939..2242
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2631..3590
                     /gene="MYOD1"
                     /label="Myoblast determination protein 1"
                     /note="Myoblast determination protein 1 from Homo sapiens.
                     Accession#: P15172"
     misc_feature    3864..4424
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4447..5163
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             5505..5945
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             6037..6270
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6342..6476
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6503..6638
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6659..6677)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6687..6703)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6848..7303
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7332..7436
                     /label="AmpR promoter"
     CDS             7437..8294
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8468..9056
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9344..9365
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9380..9410
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9418..9434
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9442..9458
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9479..9497
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"