LOCUS       V004912                 9522 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004912
VERSION     V004912
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Goncalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de
            Watering MJ, Knaan-Shanzer S, de Vries AA.
  TITLE     Genetic complementation of human muscle cells via directed stem cell
            fusion
  JOURNAL   Mol. Ther. 16 (4), 741-748 (2008)
   PUBMED   18334989
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Goncalves MA, de Vries AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands
REFERENCE   3  (bases 1 to 9522)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Ther."; date: "2008"; volume: "16"; issue: "4"; pages: "741-748"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-JUL-2007) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden 2333 ZC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1939..2242
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2631..3590
                     /gene="MYOD1"
                     /label="Myoblast determination protein 1"
                     /note="Myoblast determination protein 1 from Homo sapiens.
                     Accession#: P15172"
     misc_feature    3864..4424
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4447..5163
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             5505..5945
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             6037..6270
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6342..6476
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6503..6638
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6659..6677)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6687..6703)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6848..7303
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7332..7436
                     /label="AmpR promoter"
     CDS             7437..8294
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8468..9056
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9344..9365
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9380..9410
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9418..9434
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9442..9458
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9479..9497
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1981 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2041 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2101 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2161 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2221 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2281 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2341 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2401 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2461 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     2521 gctagaggat cccccgggct gcaggaattc ggcgcgccac gcgtaccgat ggccactctc
     2581 tgccgcttgg gttgggcgaa gccaggaccg tgccgcgcca ccgccaggat atggagctac
     2641 tgtcgccacc gctccgcgac gtagacctga cggcccccga cggctctctc tgctcctttg
     2701 ccacaacgga cgacttctat gacgacccgt gtttcgactc cccggacctg cgcttcttcg
     2761 aagacctgga cccgcgcctg atgcacgtgg gcgcgctcct gaaacccgaa gagcactcgc
     2821 acttccccgc ggcggtgcac ccggccccgg gcgcacgtga ggacgagcat gtgcgcgcgc
     2881 ccagcgggca ccaccaggcg ggccgctgcc tactgtgggc ctgcaaggcg tgcaagcgca
     2941 agaccaccaa cgccgaccgc cgcaaggccg ccaccatgcg cgagcggcgc cgcctgagca
     3001 aagtaaatga ggcctttgag acactcaagc gctgcacgtc gagcaatcca aaccagcggt
     3061 tgcccaaggt ggagatcctg cgcaacgcca tccgctatat cgagggcctg caggctctgc
     3121 tgcgcgacca ggacgccgcg ccccctggcg ccgcagccgc cttctatgcg ccgggcccgc
     3181 tgcccccggg ccgcggcggc gagcactaca gcggcgactc cgacgcgtcc agcccgcgct
     3241 ccaactgctc cgacggcatg atggactaca gcggcccccc gagcggcgcc cggcggcgga
     3301 actgctacga aggcgcctac tacaacgagg cgcccagcga acccaggccc gggaagagtg
     3361 cggcggtgtc gagcctagac tgcctgtcca gcatcgtgga gcgcatctcc accgagagcc
     3421 ctgcggcgcc cgccctcctg ctggcggacg tgccttctga gtcgcctccg cgcaggcaag
     3481 aggctgccgc ccccagcgag ggagagagca gcggcgaccc cacccagtca ccggacgccg
     3541 ccccgcagtg ccctgcgggt gcgaacccca acccgatata ccaggtgctc tgagggggat
     3601 gtggccatca tcgtcgacgc cgtatacgct ccggagcccg cggactagtc tcgagcctct
     3661 agacagcgct caccggtcgt taacattaag ctagcctagt gccatttgtt cagtggttcg
     3721 tagggctttc ccccactgtt tggctttcag ttatatggat gatgtggtat tgggggccaa
     3781 gtctgtacag catcttgagt ccctttttac cgctgttacc aattttcttt tgtctttggg
     3841 taaacccggg aattccgccc ctctcccccc ctaacgttac tggccgaagc cgcttggaat
     3901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     4021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     4081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaacggtc
     4441 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4561 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4621 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     4681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4861 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4921 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4981 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5041 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5101 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5161 aagtaaagcg gccgcgtcga gccctatggg taccatgcat gtattcaatc taagcaggct
     5221 ttcactttct cgccaactta caaggccttt ctgtgtaaac aatacctgaa cctttacccc
     5281 gttgcccggc aacggccagg tctgtgccaa gtgtttgctg acgcaacccc cactggctgg
     5341 ggcttggtca tgggccatca gcgcatgcgt ggaacctttt cggctcctct gccgatccat
     5401 actgcggaac tcctagccgc ttgttttgct cgcagcaggt ctggagcaaa cattatcggg
     5461 actgataact ctgttgtcct atcccgcaaa tatacatcgt ttccatggct gctaggctgt
     5521 gctgccaact ggatcctgcg cgggacgtcc tttgtttacg tcccgtcggc gctgaatcct
     5581 gcggacgacc cttctcgggg tcgcttggga ctctctcgtc cccttctccg tctgccgttc
     5641 cgaccgacca cggggcgcac ctctctttac gcggactccc cgtctgtgcc ttctcatctg
     5701 ccggaccgtg tgcacttcgc ttcacctctg cacgtcgcat ggagaccacc gtgaacgccc
     5761 accaaatatt gcccaaggtc ttacataaga ggactcttgg actctcagca atgtcaacga
     5821 ccgaccttga ggcatacttc aaagactgtt tgtttaaaga ctgggaggag ttgggggagg
     5881 agattaggtt aaaggtcttt gtactaggag gctgtaggca taaattggtc tgcgcaccag
     5941 caccatggcg caatcactag agcggggtac ctttaagacc aatgacttac aaggcagctg
     6001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6241 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6301 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6481 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6601 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     6661 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     6721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6841 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7261 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccaggt ggcacttttc
     7321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8581 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     8641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9481 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
