Basic Vector Information
- Vector Name:
- pLV.CAG.hrGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9413 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Pelascini LLP., Janssen JM, Goncalves MAF.V.
- Promoter:
- CAG
pLV.CAG.hrGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV.CAG.hrGFP vector Sequence
LOCUS V004916 9413 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004916
VERSION V004916
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9413)
AUTHORS Pelascini LLP., Janssen JM, Goncalves MAF.V.
TITLE Histone deacetylases hinder transgene expression from
integration-defective lentiviral vectors in dividing and
non-dividing cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9413)
AUTHORS Pelascini LLP., Janssen JM, Goncalves MAF.V.
TITLE Direct Submission
JOURNAL Submitted (06-FEB-2012) Molecular Cell Biology, Leiden University
Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC,
Netherlands
REFERENCE 3 (bases 1 to 9413)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9413)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-FEB-2012) Molecular Cell Biology, Leiden University Medical
Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9413
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 5..231
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 232..412
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 459..584
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1077..1310
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1495..1539
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1688..1729
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1801..1918
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind complement(1945..1992)
/label="FRT"
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
polyA_signal complement(2362..2417)
/label="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(2604..3320)
/label="hrGFP"
/note="humanized Renilla green fluorescent protein"
intron complement(3413..4421)
/label="chimeric intron"
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter complement(4422..4697)
/label="chicken beta-actin promoter"
enhancer 4700..5079
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
regulatory 5268..5277
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
protein_bind complement(5330..5377)
/label="FRT"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
regulatory complement(5429..5820)
/label="murine metallothionein gene polyadenylation signal"
/note="murine metallothionein gene polyadenylation signal"
/regulatory_class="polyA_signal_sequence"
LTR 5930..6163
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6235..6369
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 6396..6531
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(6552..6570)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6580..6596)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6741..7196
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7225..7329
/label="AmpR promoter"
CDS 7330..8187
/label="AmpR"
/note="beta-lactamase"
rep_origin 8361..8949
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9237..9258
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9273..9303
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 9311..9327
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9335..9351
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 9372..9390
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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