pLV.CAG.hrGFP vector (V004916)

LOCUS       V004916                 9413 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004916
VERSION     V004916
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Histone deacetylases hinder transgene expression from
            integration-defective lentiviral vectors in dividing and
            non-dividing cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2012) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC,
            Netherlands
REFERENCE   3  (bases 1 to 9413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2012) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1077..1310
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1495..1539
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1688..1729
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1801..1918
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(1945..1992)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    complement(2362..2417)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(2604..3320)
                     /label="hrGFP"
                     /note="humanized Renilla green fluorescent protein"
     intron          complement(3413..4421)
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        complement(4422..4697)
                     /label="chicken beta-actin promoter"
     enhancer        4700..5079
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5268..5277
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5330..5377)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(5429..5820)
                     /label="murine metallothionein gene polyadenylation signal"
                     /note="murine metallothionein gene polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     LTR             5930..6163
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6235..6369
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6396..6531
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6552..6570)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6580..6596)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6741..7196
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7225..7329
                     /label="AmpR promoter"
     CDS             7330..8187
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8361..8949
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9237..9258
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9273..9303
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9311..9327
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9335..9351
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9372..9390
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"