LOCUS       V004916                 9413 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004916
VERSION     V004916
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Histone deacetylases hinder transgene expression from
            integration-defective lentiviral vectors in dividing and
            non-dividing cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Pelascini LLP., Janssen JM, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2012) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC,
            Netherlands
REFERENCE   3  (bases 1 to 9413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2012) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1077..1310
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1495..1539
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1688..1729
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1801..1918
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(1945..1992)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    complement(2362..2417)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(2604..3320)
                     /label="hrGFP"
                     /note="humanized Renilla green fluorescent protein"
     intron          complement(3413..4421)
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        complement(4422..4697)
                     /label="chicken beta-actin promoter"
     enhancer        4700..5079
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5268..5277
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5330..5377)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(5429..5820)
                     /label="murine metallothionein gene polyadenylation signal"
                     /note="murine metallothionein gene polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     LTR             5930..6163
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6235..6369
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6396..6531
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6552..6570)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6580..6596)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6741..7196
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7225..7329
                     /label="AmpR promoter"
     CDS             7330..8187
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8361..8949
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9237..9258
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9273..9303
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9311..9327
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9335..9351
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9372..9390
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     1861 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     1921 cgggggatcc tctagagtca gcttgaagtt cctatacttt ctagagaata ggaacttcgg
     1981 aatagtaact tcaatgatct cgaaaatccc gggatcagct tgggctgcag gtcgagggat
     2041 cttcataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     2101 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     2161 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     2221 gggggctgtt tcatatactg atgacctctt tatagccacc tttgttcatg gcagccagca
     2281 tatggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc
     2341 cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa
     2401 aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata
     2461 atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc
     2521 agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattgatcca cgcgtaactt
     2581 aagtgcggcc gctcgagcta ttacacccac tcgtgcaggc tgcccagggg cttgcccagg
     2641 ctggtcagct gggcgatggc ggtctcgtgc tgctccacga agccgccgtc ctccacgtag
     2701 gtcttctcca ggcggtgctg gatgaagtgg tactcgggga agtccttcac cacgcccttg
     2761 ctcttcatca gggtgcgcat gtggcagctg tagaacttgc cgctgttcag gcggtacacc
     2821 aggatcacct ggcccaccag cacgccgtcg ttcatgtaca ccacctcgaa gctgggctgc
     2881 aggccggtga tggtcttctt catcacgggg ccgtcgttgg ggaagttgcg gcccttgtac
     2941 tccacgcggt acacgaacat ctcctcgatc aggttgatgt cgctgcggat ctccaccagg
     3001 ccgccgtcct cgtagcgcag ggtgcgctcg tacacgaagc cggcggggaa gctctggatg
     3061 aagaagtcgc tgatgtcctc ggggtacttg gtgaaggtgc ggttgccgta ctggaaggcg
     3121 gggctcagga tgtcgaaggc gaagggcagg ggggcgccct tggtcacgcg gatctgcacc
     3181 agctggttgc cgaacaggat gttgcccttg ccgcagccct ccatggtgaa cacgtggttg
     3241 ttcaccacgc cctccaggtt caccttgaag ctcatgatct cctgcaggcc ggtgttcttc
     3301 aggatctgct tgctcaccat ggtgaattcg tcgactctag agctctgcag gcatgcgata
     3361 attctttgcc aaaatgatga gacagcacaa caaccagcac gttgcccagg agctgtagga
     3421 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
     3481 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccctgc ggcagccccg
     3541 aggctggaga tggagaaggg gacggcggcg cggcgacgca cgaaggccct ccccgcccat
     3601 ttccttcctg ccggcgccgc accgcttcgc ccgcgcccgc tagagggggt gcggcggcgc
     3661 ctcccagatt tcggctccgc cagatttggg acaaaggaag tccctgcgcc ctctcgcacg
     3721 attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg
     3781 gccgccgcgc ccctcccccg agccctcccc ggcccgaggc ggccccgccc cgcccggcac
     3841 ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgcacggag
     3901 ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca ggggggggtt
     3961 acagcccgac cgccgcgccc acaccccctg ctcacccccc cacgcacaca ccccgcacgc
     4021 agcctttgtt cccctcgcag cccccccgca ccgcggggca ccgcccccgg ccgcgctccc
     4081 ctcgcgcaca cgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc acagccgccg
     4141 ggcagcgcgg gccgcacgcg gcgctcccca cgcacacaca cacgcacgca ccccccgagc
     4201 cgctcccccc cgcacaaagg gccctcccgg agccctttaa ggctttcacg cagccacaga
     4261 aaagaaacga gccgtcatta aaccaagcgc taattacagc ccggaggaga agggccgtcc
     4321 cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg
     4381 gcgcggagcg gggcacgggg cgaaggcaac gcagcgactc ccgcccgccg cgcgcttcgc
     4441 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     4501 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc
     4561 gccccgcctg gcgcgcgccc cccccccccc cgcccccatc gctgcacaaa ataattaaaa
     4621 aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag tgaagcagaa
     4681 cgtggggctc acctcgaccc atggtaatag cgatgactaa tacgtagatg tactgccaag
     4741 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     4801 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     4861 tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     4921 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     4981 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     5041 taattgatta ctattaataa ctagtcaata atcaatgtcg aatccatggc tcgagatttc
     5101 gaagtactca gcgtaagtga tgtccacctc gatatgtgca tctgtaaaag caattgttcc
     5161 aggaaccagg gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcttc
     5221 cagcggatag aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt
     5281 accaacagta ccggaatgcc aagcttagtg gatcaacgtt agccagcttg aagttcctat
     5341 actttctaga gaataggaac ttcggaatag taacttcaat gatcatcatg acagatctgc
     5401 gcgcgatcga taaatgtttg acctgcagag cttatgggat agcttggctt tacccaaaga
     5461 cattacccct ctgtgagggc cagaggcggg aggtaaaggc acgagggggt ccaaaaccag
     5521 atccccagac tatcaagtca ccccagtttc ttattgccaa accacaccaa gaaaaccaga
     5581 gttagactca aacaggcttt tattattcac gtactcggta gaaaacgggg gtttagtaaa
     5641 cagggtggaa ctgtatagga agacgctggg ttggtccgat actatttaca cgtggtggca
     5701 gcgctgttcg tcacatcagg cacagcacgt gcacttgtcc gcggcgcctt tgcagacaca
     5761 gccctgggca catttggagc agcccacggg acagcaggag cagcagccta gaaaggagga
     5821 caagctgtac aagtaaagcg gccgcgtcga gccctatggg tacctttaag accaatgact
     5881 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5941 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6001 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6061 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6121 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6181 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6241 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6301 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6361 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6481 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6541 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6601 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6661 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6721 cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6901 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7021 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7081 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7141 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca
     7201 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7261 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7321 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7381 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7441 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7501 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7561 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7621 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7681 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7741 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7801 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7861 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7921 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7981 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8041 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8101 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8161 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8221 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8821 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9001 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9061 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9241 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9301 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9361 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
