Basic Vector Information
- Vector Name:
- pLucGAL4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10716 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Houten SM, Volle DH, Cummins CL, Mangelsdorf DJ, Auwerx J.
pLucGAL4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLucGAL4 vector Sequence
LOCUS 40924_28836 10716 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLucGAL4, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10716)
AUTHORS Houten SM, Volle DH, Cummins CL, Mangelsdorf DJ, Auwerx J.
TITLE In vivo imaging of farnesoid X receptor activity reveals the ileum
as the primary bile acid signaling tissue
JOURNAL Mol. Endocrinol. 21 (6), 1312-1323 (2007)
PUBMED 17426284
REFERENCE 2 (bases 1 to 10716)
AUTHORS Houten SM, Auwerx J.
TITLE Direct Submission
JOURNAL Submitted (21-APR-2004) Institut de Genetique et de Biologie
Moleculaire et Cellulaire, 1 rue Laurent Fries, Illkirch 67404,
France
REFERENCE 3 (bases 1 to 10716)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10716)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Endocrinol."; date: "2007"; volume: "21"; issue: "6"; pages:
"1312-1323"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-APR-2004) Institut de Genetique et de Biologie Moleculaire et
Cellulaire, 1 rue Laurent Fries, Illkirch 67404, France"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10716
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(24..40)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 181..636
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 714..818
/label=AmpR promoter
CDS 819..1676
/label=AmpR
/note="beta-lactamase"
rep_origin 1850..2438
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2726..2747
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2762..2792
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2800..2816
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2824..2840
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2858..2876
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 2902..4124
/label=beta-globin insulator
/note="beta-globin insulator"
misc_feature 4125..5334
/label=beta-globin insulator
/note="beta-globin insulator"
protein_bind 5385..5479
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
regulatory 5522..6631
/label=Wnt-1 promoter
/note="Wnt-1 promoter"
/regulatory_class="promoter"
CDS 6673..8322
/label=luciferase
/note="firefly luciferase"
polyA_signal complement(8366..8487)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 8601..10689
/label=3' flanking sequence of Wnt-1
/note="3' flanking sequence of Wnt-1"
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