LOCUS       40924_28836       10716 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLucGAL4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Houten SM, Volle DH, Cummins CL, Mangelsdorf DJ, Auwerx J.
  TITLE     In vivo imaging of farnesoid X receptor activity reveals the ileum 
            as the primary bile acid signaling tissue
  JOURNAL   Mol. Endocrinol. 21 (6), 1312-1323 (2007)
  PUBMED    17426284
REFERENCE   2  (bases 1 to 10716)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2004) Institut de Genetique et de Biologie 
            Moleculaire et Cellulaire, 1 rue Laurent Fries, Illkirch 67404, 
            France
REFERENCE   3  (bases 1 to 10716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Endocrinol."; date: "2007"; volume: "21"; issue: "6"; pages: 
            "1312-1323"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2004) Institut de Genetique et de Biologie Moleculaire et 
            Cellulaire, 1 rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      181..636
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /label=AmpR promoter
     CDS             819..1676
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1850..2438
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2902..4124
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     misc_feature    4125..5334
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     protein_bind    5385..5479
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      5522..6631
                     /label=Wnt-1 promoter
                     /note="Wnt-1 promoter"
                     /regulatory_class="promoter"
     CDS             6673..8322
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(8366..8487)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8601..10689
                     /label=3' flanking sequence of Wnt-1
                     /note="3' flanking sequence of Wnt-1"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gcgacgcgtc gactctagag ccccatcctc actgactccg
     2941 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     3001 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     3061 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     3121 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     3181 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     3241 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     3301 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     3361 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     3421 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     3481 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     3541 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     3601 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     3661 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     3721 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     3781 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     3841 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     3901 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3961 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4021 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4081 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc
     4141 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     4201 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     4261 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     4321 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     4381 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     4441 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     4501 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     4561 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     4621 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     4681 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     4741 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     4801 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     4861 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     4921 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     4981 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     5041 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     5101 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5161 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     5221 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     5281 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     5341 ttaactacgt acggccgcgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc
     5401 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     5461 cggagtactg tcctccgagc ggagactcta gcgagcgccg gaggcggccg ctcgactcat
     5521 cacgtctcct gtcacagccc cacctccttc ctgatacact gcgttaagtg gtgactgtaa
     5581 caaaatgact tcatgctctc cctgtcctga gccaaattac acaattattt ggaaagggct
     5641 caaaatgttc ttcgttagaa gtttctggat acaccaatac acaggagcgt gcaccctcag
     5701 aacacatgta cactttgact taatctcacg ggtgacacac cgacgcttac actcccccta
     5761 gcccacagag gcaaactgct gggcgcttct gagtttctca ctgccaccag ctcggtttgc
     5821 tcagcctacc cccgcacccc gcgcccggga atccctgacc acagctccac ccatgctctg
     5881 tctccttctt ttccttctct gtccagccgt cggggttcct gggtgaggaa gtgtctccac
     5941 ggagtcgctg gctagaacca caactttcat cctgccattc agaataggga agagaagaga
     6001 ccacagcgta ggggggacag aggagacgga cttcgagagg acagccccac cggcgcgtgt
     6061 gggggaggca atccaggctg caaacaggtt gtccccagcg cattgtcccc gcgccccctg
     6121 gcggatgctg gtccccgacg ggctccggac gcgcagaaga gtgaggccgg cgcgcgtggg
     6181 aggccatccc aaggggaggg gtcggcggcc agtgcagacc tggaggcggg gccaccaggc
     6241 agggggcggg ggtgagcccc gacggttagc ctgtcagctc tttgctcaga ccggcaagag
     6301 ccacagcttc gctcgccact cattgtctgt ggccctgacc agtgcgccct ggtgctttta
     6361 gtgccgcccg ggcccggagg ggcagcctct tctcactgca gtcagcgccg caactataag
     6421 aggcctataa gaggcggtgc ctcccgcagt ggctgcttca gcccagcagc caggacagcg
     6481 aaccatgctg cctgcggccc gcctccagac ttattagagc cagcctggga actcgcatca
     6541 ctgccctcac cgctgtgtcc agtcccaccg tcgcggacag caaccacagt cgtcagaacc
     6601 gcagcacaga accagcaagg ccaggcaggc ctcgcgatag cttggcattc cggtactgtt
     6661 ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     6721 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     6781 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     6841 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     6901 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     6961 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     7021 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     7081 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     7141 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     7201 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     7261 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     7321 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     7381 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     7441 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     7501 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     7561 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     7621 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     7681 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     7741 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     7801 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     7861 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     7921 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     7981 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     8041 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     8101 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     8161 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     8221 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     8281 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc
     8341 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     8401 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     8461 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     8521 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     8581 atcgataagg atccgtcgat tttggtcgcc tctttggccg agagttcgtg gactccgggg
     8641 agaaggggcg ggacctacgc ttcctcatga accttcacaa caacgaggca gggcgaacgg
     8701 tacgtcggtg tgtccggaac caatggcagg ggagatgtaa gacaggtgca cggggacaga
     8761 ggcacaggga ggggcttccc gagagagtgg gactctagga gggaagacag agaagaggtg
     8821 gtggttgagg gcaaagaggt tcctgagctg atgacagaac agaagagatt agcaggctat
     8881 caacacgtgg gatgtattga gatggctcca tggcacactt ttgaaagata aaagtgactt
     8941 gctggcgtgg agcagagtct ggccgaatgt ccctatctca gcgggccatt ttgcacttcc
     9001 tctctcccga gcttagtcac acctggacct tggctgaagt ttccacagca tcgacgtgac
     9061 ccgggtgggg tgggggtggg gaagtatggg tggtggttcg tgggatgttg gctttgacct
     9121 tttcttccct cctcccctcg tcccctcctc ccccagaccg tgttctctga gatgcgccaa
     9181 gagtgcaaat gccacgggat gtccggctcc tgcacggtgc gcacgtgttg gatgcggctg
     9241 cccacgctgc gcgctgtggg cgacgtgctg cgcgaccgct tcgacggcgc ctcccgcgtc
     9301 ctttacggca accgaggcag caaccgcgcc tcgcgggcgg agctgctgcg cctggagccc
     9361 gaagaccccg cgcacaagcc tccctcccct cacgacctcg tctacttcga gaaatcgccc
     9421 aacttctgca cgtacagtgg ccgcctgggc acagctggca cagctggacg agcttgcaac
     9481 agctcgtctc ccgcgctgga cggctgtgag ctgctgtgct gtggccgagg ccaccgcacg
     9541 cgcacgcagc gcgtcacgga gcgctgcaac tgcaccttcc actggtgctg ccacgtcagc
     9601 tgccgcaact gcacgcacac gcgcgttctg cacgagtgtc tatgaggtgc cgcgcctccg
     9661 ggaacgggaa cgctctcttc cagttctcag acacactcgc tggtcctgat gtttgcccac
     9721 cctaccgcgt ccagccacag tcccagggtt catagcgatc catctctccc acctcctacc
     9781 tggggactcc tgaaaccact tgcctgagtc ggctcgaacc cttttgccat cctgagggcc
     9841 ctgacccagc ctacctccct ccctctttga gggagactcc ttttgcactg ccccccaatt
     9901 tggccagagg gtgagagaaa gattcttctt ctggggtggg ggtggggagg tcaactcttg
     9961 aaggtgttgc ggttcctgat gtattttgcg ctgtgacctc tttgggtatt atcacctttc
    10021 cttgtctctc gggtccctat aggtcccttg agttctctaa ccagcacctc tgggcttcaa
    10081 ggcctttccc ctcccacctg tagctgaaga gtttccgagt tgaaagggca cggaaagcta
    10141 agtgggaaag gaggttgctg gacccagcag caaaacccta cattctcctt gtctctgcct
    10201 cggagccatt gaacagctgt gaaccatgcc tccctcagcc tcctcccacc ccttcctgtc
    10261 ctgcctcctc atcactgtgt aaataatttg caccgaaatg tggccgcaga gccacgcgtt
    10321 cggttatgta aataaaacta tttattgtgc tgggttccag cctgggttgc agagaccacc
    10381 ctcaccccac ctcactgctc ctctgttctg ctcgccagtc cttttgttat ccgacctttt
    10441 ttctctttta cccagcttct cataggcgcc cttgcccacc ggatcagtat ttccttccac
    10501 tgtagctatt agtggctcct cgcccccacc aatgtagtat cttcctctga ggaataaaat
    10561 atctattttt atcaacgact ctggtccttg aatccagaac acagcatggc ttccaacgtc
    10621 ctcttccctt ccaatggact tgcttctctt ctcatagcca aacaaaagag atagagttgt
    10681 tgaagatctg tcgacgacgt cgggcccaat tcgccc
//
