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pLT_03 vector (V004927)

Basic Vector Information

Vector Name:
pLT_03
Antibiotic Resistance:
Ampicillin
Length:
5242 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Tian L.

pLT_03 vector Map

Copy Sequence View Map
pLT_035242 bp6001200180024003000360042004800oriAmpRAmpR promotermisc_recomb

pLT_03 vector Sequence

LOCUS       V004927                 5242 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004927
VERSION     V004927
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Tian L.
  TITLE     Methods for metabolic engineering of Thermoanaerobacterium
            saccharolyticum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(763..1620)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1621..1725)
                     /label="AmpR promoter"
     CDS             3321..4055
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_recomb     4018..5067

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