LOCUS       V004927                 5242 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004927
VERSION     V004927
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Tian L.
  TITLE     Methods for metabolic engineering of Thermoanaerobacterium
            saccharolyticum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(763..1620)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1621..1725)
                     /label="AmpR promoter"
     CDS             3321..4055
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_recomb     4018..5067
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg actgcgaaga taagatgtta agtggtgaag gtgtgatgaa taacgggata
     1861 atttcaacaa aaattggttt acggggaata ccacatgaag cggaagaggt aatgttggcg
     1921 cgaaatatta tattggcgaa atctactgga gcaaaacttc atgtagcaca tgtatcaaca
     1981 aaggatagtg ttgaattgat aagaagagct aaggcggacg gagtagatat aacggcagaa
     2041 gtaacaccac acaatctatg ccttactgaa gatatggttg atggctttga cacgaataca
     2101 aaggcttatc ctccattaag gacaaaagag gatgtagatg ctcttataga gggattaaaa
     2161 gatggcacca tagatgttat tgcaacagat catgctccac atacgaaaga tgataaaaaa
     2221 gttccttatg agatagcggc ttttgggata tcaggaatcg aaatcgcctt ttctgtagtg
     2281 tatacgtacc ttgttaaaaa tggattgatt gcgatagacg atttggtaaa acgcatgtca
     2341 atagtacctt cgaaactatt aaaattaacc catggcttta agatagggaa tgatgcagat
     2401 atgacaatag tagatttagg caaagaatat acagtcgatg tagacaattt taaatcaaaa
     2461 ggcaaaaatt caccatttaa tggacaaaaa ttgtacggag tggtggaata tacgattgta
     2521 aatggagaaa taaaatttaa aaatgatggc aattatcgag taagtaatgt ataatatata
     2581 tagaataatt atacattgat atgaataaat atttataaga ggtgttaata tgttttcgga
     2641 taatttgata catgcaataa aattcaaaaa taatcccacg gttgtcggtt tggagcattc
     2701 gagatattaa aaagttgtta atacaccgaa aggtgtttaa tataaaaata aaaattaaag
     2761 gaggtgaaat ggtcgataaa cccagcgaac catttgaggt gataggtaag attataccga
     2821 ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa
     2881 gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca
     2941 atactgataa gataatatat cttttatata gaagatatcg ccgtatgtaa ggatttcagg
     3001 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     3061 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     3121 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tcaaaacaac
     3181 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     3241 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     3301 aataataaat ggcggatccc atgaacaaaa atataaaata ttctcaaaac tttttaacga
     3361 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
     3421 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
     3481 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
     3541 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
     3601 ggtataaaat tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg
     3661 tttttgaaag ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
     3721 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
     3781 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
     3841 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
     3901 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
     3961 caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta
     4021 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa
     4081 tttggaaagt tacacgttac taaagggtgg gttgtggaat tggtgcgtgt atggtatgtg
     4141 cctgtaaaac aaaaactgat tctggatttc aatataaaaa agtctgtaag gatggtcctg
     4201 tattttggtc aagggaggta gtattttgaa tctgaaagtc aatataggaa accttgaact
     4261 taaaaatcca gttcttgtgg catctggcac atttggcttt gggagagagt attcacatta
     4321 tatcgattta gatgatttag gaggaataat ggtaaagggg cttacattgt atccaaaaga
     4381 aggaaatcct ccaccgcgta tatacgaaac gccatcagga attttaaata gcgttgggct
     4441 gcaaaatcca ggtgtcgatg cattcataaa agatgaattg ccatttttaa ataggtttaa
     4501 tactaaagtg atcgcaaata tagcaggaga gacaatagaa gaatttgtat cattagccca
     4561 aaaacttaat atagacggtg tagatgcatt agagatcaat gtttcatgcc caaatgtaaa
     4621 agaaggaggg atgtcgtttg gagtagatcc caacagtgta tatgcgataa ctaaaaaagt
     4681 aaaacaagca tcaaacaaaa cagtaattgt aaaattgact ccaaatgtaa ctgacataaa
     4741 gatttatgct aaagcagcag aagatggtgg tgctgatgca atttctctta taaatacagt
     4801 tactggaatg gctatagaca taaacacaag gcgacctgtt ttcaagaatg tgtttgctgg
     4861 tttatcagga ccagctataa aacctatagc gcttaaaaat gtgtatgaag ctaaaagagc
     4921 tgtatcaata cctgtgatag ggatgggagg aatttcatct gcagaagatg ctttagaatt
     4981 tatgatagca ggggccactg ctgtagccgt tggaacttat aattttatca atcccgccgg
     5041 ttcaatagac attttagaag gcataaactt cctcgctcac tgactcgctg cgctcggtcg
     5101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5221 aaaaggccgc gttgctggcg tt
//
