pLTSUB-302 vector (V004928)

LOCUS       40924_28801        4719 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Low threshold vector pLTSUB-302, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Basu S, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2005) Electrical Engineering, Princeton 
            University, J-319, E-quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005"; volume: "434"; issue: "7037"; pages: "1130-1134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAR-2005) Electrical Engineering, Princeton University, J-319, 
            E-quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..74
                     /note="Luxp(r); Lux promoter"
                     /regulatory_class="promoter"
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             115..825
                     /label=lambda repressor
                     /note="phage lambda repressor"
     RBS             886..897
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             904..1620
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      1647..1733
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     RBS             1848..1859
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1866..2945
                     /label=lacI
                     /note="lac repressor"
     CDS             3103..3894
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      3928..4022
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4136..4681
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."