LOCUS       40924_28801        4719 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Low threshold vector pLTSUB-302, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Basu S, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2005) Electrical Engineering, Princeton 
            University, J-319, E-quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005"; volume: "434"; issue: "7037"; pages: "1130-1134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAR-2005) Electrical Engineering, Princeton University, J-319, 
            E-quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..74
                     /note="Luxp(r); Lux promoter"
                     /regulatory_class="promoter"
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             115..825
                     /label=lambda repressor
                     /note="phage lambda repressor"
     RBS             886..897
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             904..1620
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      1647..1733
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     RBS             1848..1859
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1866..2945
                     /label=lacI
                     /note="lac repressor"
     CDS             3103..3894
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      3928..4022
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4136..4681
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 caattactta acataagcac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt
       61 atagtcgaaa gagaatttca cacaggggta ccaattaaag aggagaaatt aagcatgagc
      121 acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt
      181 tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg
      241 atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac
      301 gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aattcagccc ttcaatcgcc
      361 agagagatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat
      421 gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt
      481 accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc
      541 tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct
      601 gacggaatgt taattctcgt tgaccctgag caggctgttg agcccgggga tttctgcata
      661 gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg
      721 tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt
      781 gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctgatc ggcaaggtgt
      841 tctggtcggc gcatagctga taacaattgg tacctagaat tgcttaaaga ggagaaatta
      901 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      961 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1081 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg
     1141 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1321 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag
     1381 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1501 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1561 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1621 taatcggccg tctcgagtag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1681 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     1741 cctagaccta gtagcacgac aggttcctag taaatatcta acaccgtgcg tgttgactat
     1801 tttacctctg gcggtgataa tggttgcatg tagctaccga attcagcaaa gaggagaaat
     1861 taagcgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     1921 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     1981 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     2041 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     2101 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     2161 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     2221 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     2281 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     2341 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     2401 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     2461 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     2521 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     2581 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     2641 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     2701 gctcatgtta tatcccgccg tcaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     2761 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     2821 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     2881 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2941 ggcagtgagc gcaacgcaat gacgtcggaa ttgccagctg gggcgccctc tggtaaggtt
     3001 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     3061 ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     3121 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3181 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3241 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     3301 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     3361 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     3421 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     3481 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     3541 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     3601 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     3661 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     3721 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     3781 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     3841 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     3901 ggactctggg gttcgagagc tcgcttggac tcctgttgat agatccagta atgacctcag
     3961 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     4021 atccaagcac tagtaacaac ttatatcgta tggggctgac ttcaggtgct acatttgaag
     4081 agataaattg cactgaaatc tagtaatatt ttatctgatt aataagatga tcttcttgag
     4141 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
     4201 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
     4261 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
     4321 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
     4381 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
     4441 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     4501 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     4561 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4621 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     4681 aaacggcttt acgttcacac tattgtatcg ctgggaata
//
