pLPV111 vector (V004952)

LOCUS       V004952                 4235 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004952
VERSION     V004952
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Axelsson L, Holck A.
  TITLE     The genes involved in production of and immunity to sakacin A, a
            bacteriocin from Lactobacillus sake Lb706
  JOURNAL   J. Bacteriol. 177 (8), 2125-2137 (1995)
   PUBMED   7721704
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L.
  TITLE     Plasmid p256 from Lactobacillus plantarum represents a new type of
            replicon in lactic acid bacteria, and contains a
            toxin-antitoxin-like plasmid maintenance system
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)
   PUBMED   15699191
REFERENCE   3  (bases 1 to 4235)
  AUTHORS   Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Microbiology, MATFORSK, Osloveien
            1, Aas N-1430, Norway
REFERENCE   4  (bases 1 to 4235)
  AUTHORS   Skaugen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   5  (bases 1 to 4235)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1995"; volume: "177"; issue: "8"; pages:
            "2125-2137"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Microbiology, MATFORSK, Osloveien 1, Aas
            N-1430, Norway"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..110
                     /label="multicloning site"
                     /note="multicloning site"
     promoter        complement(122..140)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(158..174)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(182..198)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(206..236)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(251..272)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(560..1148)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1682..2416)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(2513..2596)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77688.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(2513..2596)
                     /gene="ermBL"
                     /label="ermBL"
     misc_feature    2755..3635
                     /note="p256 replication region; allows replication in
                     Lactobacillus species"
     rep_origin      complement(3675..4055)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4196..4212
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4219..4235
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"