LOCUS       V004952                 4235 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004952
VERSION     V004952
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Axelsson L, Holck A.
  TITLE     The genes involved in production of and immunity to sakacin A, a
            bacteriocin from Lactobacillus sake Lb706
  JOURNAL   J. Bacteriol. 177 (8), 2125-2137 (1995)
   PUBMED   7721704
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L.
  TITLE     Plasmid p256 from Lactobacillus plantarum represents a new type of
            replicon in lactic acid bacteria, and contains a
            toxin-antitoxin-like plasmid maintenance system
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)
   PUBMED   15699191
REFERENCE   3  (bases 1 to 4235)
  AUTHORS   Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Microbiology, MATFORSK, Osloveien
            1, Aas N-1430, Norway
REFERENCE   4  (bases 1 to 4235)
  AUTHORS   Skaugen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   5  (bases 1 to 4235)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1995"; volume: "177"; issue: "8"; pages:
            "2125-2137"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Microbiology, MATFORSK, Osloveien 1, Aas
            N-1430, Norway"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..110
                     /label="multicloning site"
                     /note="multicloning site"
     promoter        complement(122..140)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(158..174)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(182..198)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(206..236)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(251..272)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(560..1148)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1682..2416)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(2513..2596)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77688.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(2513..2596)
                     /gene="ermBL"
                     /label="ermBL"
     misc_feature    2755..3635
                     /note="p256 replication region; allows replication in
                     Lactobacillus species"
     rep_origin      complement(3675..4055)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4196..4212
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4219..4235
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc tctagactcg aggaattcgg taccccgggt
       61 tcgaaatcga taagcttgga tccggagagc tcccaacgcg ttggatgcat agcttgagta
      121 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      541 aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc
      601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1501 agccagccgg aagggccgag cgcagaagtg gtccgatttc gaaccccgat acaaattccc
     1561 cgtaggcgct agggacctct ttagcttctt ggaagctgtc agtagtatat ctaataattt
     1621 atctccattc cctttagtaa cgtgtaactt tccaaattta aaaaagcgac tcatagaatt
     1681 atttcctccc gttaaataat agataactat taaaaataga caatacttgc tcataagtaa
     1741 tggtacttaa attgtttact ttggcgtgtt tcattgcttg acgaaactga tttttagtaa
     1801 acagttgacg atattctcga ttgacccatt ttgaaacaaa gtacttatat agcttccaat
     1861 atttatctgg aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg
     1921 gtttaggatg aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt
     1981 gcaagagcaa ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa
     2041 cagtcagata gatgtcagac gcacggcttt caaaaaccac ttttttaata atttgtgtgc
     2101 ttaaattgta aggaatattc ccaacaattt tatacctctg tttgttaggg aattgaaact
     2161 gtagaatatc ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata
     2221 agttgaatag atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt
     2281 tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat
     2341 tcaattgttt tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt
     2401 ttatattttt gttcatgtaa tctctcctga agttaaacga tttgtttgta attttagtta
     2461 tctgtttaaa aagtcataag attagtcact ggtaggaatt aatctaacgt atttatttat
     2521 ctgcgtaatc actgttttta gtctgtttca aaacagtaga tgttttatct acattacgca
     2581 tttggaatac caacatgacg aatccctcct tcttaattac aaatttttag catctaattt
     2641 aacttcaatt cctattttta gcatctaatt taacttcaat tcctattata caaaatttta
     2701 agataatgca ctatcaacac actcttaagt ttgcttctaa ggggtaccga attcctagta
     2761 tattaaataa caccttaagt ctagcaccaa cccgcacgca tagcggtgct taaaccatca
     2821 agggtcaagc ccttaggctc tctcaaacag ttatcctaat cgtgaataac tgcgcttctt
     2881 ttgcagtata aagagagaac tctttatcag acaatttaag ctcaaccagc ccttgcacta
     2941 actattatta gagttggttt tagcagcaac ccgaataatc tgcgttaata gttagcctgt
     3001 ccgtatcatt tcctagtctt ccagccacgt ctttagtcgc gttgatctcg acaaggttta
     3061 gcatacctat gttgttaact gcaagcgggg tcacgaacga cactcacggg aggttttact
     3121 agctaagaac aggtttccag cctttagttg ctttgatggt tgctaaccat tgaataacaa
     3181 aaaaacggtt gctatcaggt ttctgttaag attcccgata acaaccgttt actttaagta
     3241 tcaatggttg aaaaacttag cctacatgtt ataatagtac caagttagat agcttgtatt
     3301 ggtagtactt gctatcgaaa atcttatcag gttgtgctga taagtcgtga atcctaactc
     3361 tgctaagttg agggttcttt tttttgcgtt catttattaa gttgagtaca ttataaccgt
     3421 aatataagat taatacaacc tttatcattt taacgtctca accagccgaa taatcctttt
     3481 ttaagcgttt tagtttcttc ccccttgtaa tgatccccag tgggttctaa aacgtctgta
     3541 tcgtcctttg aggcgtcctc gctggtcttt tgtggggcat cgagtagttt ttcattttga
     3601 cggttagtgg ataagtttaa ttgctgttgc tgatcctcta atttgttaaa tcagctcatt
     3661 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3721 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3781 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
     3841 atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc
     3901 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3961 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     4021 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     4081 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     4141 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     4201 acgacggcca gtgaattgta atacgactca ctata
//
