pLMF04 vector (V004970)

LOCUS       V004970                 6714 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004970
VERSION     V004970
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu,
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6714)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAR-2016) Institute of Preventive Veterinary Medicine, Sichuan
            Agricultural University, No. 211, Huimin Road, Chengdu, Sichuan
            611130, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    290..311
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        326..356
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2671..2688
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3360..4322)
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
     primer_bind     complement(4502..4518)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4992..5096
                     /label="AmpR promoter"
     CDS             5097..5954
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6128..6714
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"