LOCUS       V004970                 6714 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004970
VERSION     V004970
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu,
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6714)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAR-2016) Institute of Preventive Veterinary Medicine, Sichuan
            Agricultural University, No. 211, Huimin Road, Chengdu, Sichuan
            611130, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    290..311
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        326..356
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2671..2688
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3360..4322)
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
     primer_bind     complement(4502..4518)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4992..5096
                     /label="AmpR promoter"
     CDS             5097..5954
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6128..6714
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      421 ttgggctatt taggcattag ccctcttttt aacctgactt aaaaaataat taaatcataa
      481 gatattaaat atcaagtgat tatgatttac tatgctatac ataatatcac ccagtgttag
      541 aaaattcttg ctatgctttt tgtttgtttt ttttcattcg tgaactttta gaatactttt
      601 aaaataaact caaatttata aactattgat tttcaatatt atacaaaacc aaacttatta
      661 acattaaact tatttctatt acctttaaaa taaactacta aaagacaaat ctaccaatcc
      721 tcggaactat ttaataaata ggaagttata taaaaataat gaacttttag aatactttat
      781 tagtcttttt agctccaaaa atgaactttt agaatacttt ttttgaactt ttagaatact
      841 tttttaaact tgctttttag taagttcatt ttttatatat ttgtattcta aaagtgcatt
      901 ttatattcat aactctctat atatggcaaa agtagataat aaactaatgt ttcagtccta
      961 tatttttact acggctaaat atgatttttc agtctatgaa aagcgtatac tatataggca
     1021 aatagaaata gaacaggcat tgctaaataa tgaggcgttg aaagattgta tcaaaataga
     1081 tactaaccta tggggggata aaaaatatac aattccaatt tctatgctgt tagctaatga
     1141 tgaagataaa aattattata aaattaaaaa agcattcaaa gacctaaaat caaagaacat
     1201 tgagtacgaa gatgaaaaag aatacgcttg ctttggtgtt attgataagt tttggattga
     1261 taaatatgga agaaatgtta cttggcaatc tgacaaaaga attgttgaag cggtaatgga
     1321 ttttacaaga ggatggagaa aatatgaatt gaagattgct atgcagtttg agactattta
     1381 tgcaatgcgt ttttacgaac tgatagctaa taaaaccaaa ccaataacat atgaagtgtc
     1441 atttcttgaa aaaatgtttc aattggaaaa taagtacagg aaccctcagg gtaatcttaa
     1501 tatcagtatg tttaaacgcc gtgtattaga ccccgctaaa aacgagttag ataaatgttc
     1561 tccatacact tttgattata ctctttcaaa ggataaaaaa attatttctc taattcctat
     1621 tttccaagaa caatttgcag atgaaagtat gaagatgatc aaaatagaag aggaaaaaga
     1681 tatacactct gtattaactc aaagagaaat tgatgttttt acaaacgaat ttggttttac
     1741 acaacaaggt ttattaaaca actatgccct attcaaagat tgtaaggcta cacttccatc
     1801 aaactattct ttcttccttt ttcaagagat aagaaaaagt cggactaaga aaggaaaaat
     1861 atctcctgct tatgtgattg ggattataag aaatatgctt aaagatttca aaaaagaaca
     1921 acaataggct tatgaaaaaa tatacaacaa aagaaatcgc agagcttttt ggcgtcagcg
     1981 aacggacaat ccagcgacac ataacgacac taagcgacaa catatcaaat accgacagaa
     2041 agggcttttc aataccagaa gacattgttt tctttcttgc cgagcgacac cattacgaca
     2101 ttttagcgac aacgaacgac aacgaacgac aggataaaaa tgaagaattt cctcatattg
     2161 aatacttcac agaagaggaa taccaagagt ttaaaaaaag gatcacagag tacccttttt
     2221 tgaaagagca aattaaactg tctaatgaac atttggatac tctaaaaaca caaatagaat
     2281 atttcagact atcttacaat aagcaattgg atattcacga aaaattaatc ggagcgttcc
     2341 gagaaagaaa ctttattgaa gctaaagaaa aggggatgga taaatagctc ctaataatta
     2401 aagaagtatt agaaaaaaaa tgctatataa aaacttaatc gagctctata cgagatctgt
     2461 tccaatgcat tggctgcagg tcgacattag taggtactca gaaaaaatct tctggtaata
     2521 aaagaagaga aagaaaataa tttaattaga gttagaagat agaaattaga aattagatct
     2581 tccgttacaa gagctgaatt taattttttt caatctaaca tctaacatct aacatctaat
     2641 atataaaatt ccatggctct agaactcgag catcatcatc atcatcattg aactagtgga
     2701 gttgcttctc ttttcgggag tggaaaactg aaagaactgg aacgtgccaa cgaaaaactg
     2761 caagacgagg tttcaaaacg gaacaccaat attgaaaaat tgcagagcca agtacagcag
     2821 atgcagaaac agcatgatac gcaaatccac aatctcagag aaatgcacag gcaggaactt
     2881 gacatgaaag aaaaagaact gtcacggctc gccagaatca tagacaaggc tttcaggtgg
     2941 tttccgatgt tcagggaaat gctgcgcatg gaaaagtttt gtgccatgct gggattctct
     3001 aaagaaatga ctgaaagtct tatagtcaaa aaagaagccc tgaaatgtag cggtaaaatc
     3061 tattccgagc aacacaggcg gaactttgat ataaaggatg atattttaag ggtggaaaat
     3121 gaccctgacg atgaaagcag gctgaacctg acaataaaca ggaagccgat tgccgactgg
     3181 ttcagggagc aatggcacag gcttagatat ggagcaagag tgccgcaaca ggaagaaaga
     3241 aaaagtagag gattcaaatt ataatagaag caatttgatt agtaatctaa aagcactccg
     3301 ataacgatta gagtgctttt agattgttta tcattaatta tcaaagcaag tgcagtttaa
     3361 gattttactg aagtttgcat taataaagaa tatactacag ctgatatatg cgcaacatat
     3421 tgtgacgctt gtgatttatt tcccttgaaa tccttaacaa ataccgctaa ggtataactg
     3481 atattattag gcagacatat ataggcaaca tcattgtgag ctgcaagaac accattttca
     3541 ttaacataac ctgaacctgt cttatgcgct ataacaaccc cttctttatc aagaagtgga
     3601 gctgctatcc tatctacacc tgttttgcat tcttttaacg tattcttaat gaaactttgt
     3661 ttctcatcat cgataagacc ttcagtaaac aaacgattca tcaacattgc agcaccaaga
     3721 ggagatgtat agttagagta agccttgtta tggtcagccg acatttcctc ttccgtataa
     3781 gctatctgaa aacttgaacg aggaatgagt gtggctataa aactatctgt ttgagcgaca
     3841 ttaaccatat ccttaaacat aaggttgctt gcattgttgt cactctgagt aagagtataa
     3901 cgcagcaaat ctctcactgt caatgatatg actggccctg aataatcttt cagcatagga
     3961 ctccaagtct ttgggtcaag tttatcccta tttatattta ctaaggtatc aagtgaaatt
     4021 cctttattgt caaagtcatt acaaagagct aatgcctgat gaaccttaaa cacactcatc
     4081 ataggataaa cactcttatt attgacctta accgtatctc tgttattaac aataaccgcc
     4141 acaccaattt cgccaggaca agctgagaca atttgagaaa tgctatcagt caaaacattt
     4201 gttaaaggag gatttgcgct atcttttgtc gctgatttat ggaacaatga aaataccaag
     4261 atgaaaatgc aaactaaagc tatactcaaa actacgattt gtttttttct gtttttttcc
     4321 atgttttata ttatttatat ttgtttgacg agaatatctt tatttgccga caaaggtaca
     4381 taactaaagt ttcccaccca aataaataga tagaaaaata acagtttgtc gaattttctt
     4441 tgtaaattag taatcgctaa agaactgatt ttggggatcc ccgggtaccg agctcgaatt
     4501 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4561 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4621 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     4681 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4741 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4801 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4861 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4921 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4981 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5041 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5101 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5161 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5221 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5281 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5341 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5401 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5461 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5521 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5581 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5641 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5701 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5761 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5821 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5881 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5941 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6001 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6061 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tgga
//
