pLL102 vector (V004992)

LOCUS       V004992                 6563 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004992
VERSION     V004992
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Lei MG, Cue D, Alba J, Junecko J, Graham JW, Lee CY.
  TITLE     A single copy integration vector that integrates at an engineered
            site on the Staphylococcus aureus chromosome
  JOURNAL   BMC Res Notes 5 (1), 5 (2012)
   PUBMED   22221385
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Lee CY.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2011) Microgiology and Immunology, University of
            Arkansas for Medical Sciences, 4301 W. Markham St. slot 511, Little
            Rock, AR 72205, USA
REFERENCE   3  (bases 1 to 6563)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6563)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2012"; volume: "5"; issue: "1"; pages: "5"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-SEP-2011) Microgiology and Immunology, University of Arkansas
            for Medical Sciences, 4301 W. Markham St. slot 511, Little Rock, AR
            72205, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(715..2091)
                     /gene="tet"
                     /label="Tetracycline resistance protein"
                     /note="Tetracycline resistance protein from Staphylococcus
                     aureus. Accession#: P02983"
     misc_feature    2459..2476
                     /label="attP2 core sequence"
                     /note="attP2 core sequence"
     CDS             complement(2822..3610)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             complement(4865..5812)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(5860..6082)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"