LOCUS       V004992                 6563 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004992
VERSION     V004992
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Lei MG, Cue D, Alba J, Junecko J, Graham JW, Lee CY.
  TITLE     A single copy integration vector that integrates at an engineered
            site on the Staphylococcus aureus chromosome
  JOURNAL   BMC Res Notes 5 (1), 5 (2012)
   PUBMED   22221385
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Lee CY.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2011) Microgiology and Immunology, University of
            Arkansas for Medical Sciences, 4301 W. Markham St. slot 511, Little
            Rock, AR 72205, USA
REFERENCE   3  (bases 1 to 6563)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6563)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2012"; volume: "5"; issue: "1"; pages: "5"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-SEP-2011) Microgiology and Immunology, University of Arkansas
            for Medical Sciences, 4301 W. Markham St. slot 511, Little Rock, AR
            72205, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(715..2091)
                     /gene="tet"
                     /label="Tetracycline resistance protein"
                     /note="Tetracycline resistance protein from Staphylococcus
                     aureus. Accession#: P02983"
     misc_feature    2459..2476
                     /label="attP2 core sequence"
                     /note="attP2 core sequence"
     CDS             complement(2822..3610)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             complement(4865..5812)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(5860..6082)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt
       61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
      121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac
      181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg
      241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt
      301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat
      361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta
      421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat
      481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc
      541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt
      601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
      661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
      721 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat
      781 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac
      841 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc
      901 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga
      961 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac
     1021 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa
     1081 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc
     1141 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac
     1201 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat
     1261 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa
     1321 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt
     1381 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt
     1441 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt
     1501 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt
     1561 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat
     1621 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc
     1681 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc
     1741 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat
     1801 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat
     1861 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata
     1921 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg
     1981 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca
     2041 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc
     2101 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc
     2161 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat
     2221 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc
     2281 caaaaatcga taatttagga ttgtggttat tttttgcgtt tttttggggc aaaaaaaggg
     2341 cagattattt gaaaaagggc aaacgcttgt ggaaaagcta aaaggttaaa aatgacaaaa
     2401 accttgatac aacagtgttt ttggacgctc gtgtacgtta gagaatgacc ggtttaccat
     2461 cgtgtcacga tgggattaac ttgtgttaaa aagcctttaa tatcagttgt tacaaaggat
     2521 ttgtagcgtc tttaaaaata aaaaagggca gaaaaagggc agataccttt tagtagatct
     2581 ggatctggat tttaatgcgg atgttgcgat tacttcgcca actattgcga taacaagaaa
     2641 aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa
     2701 ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc
     2761 taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt
     2821 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     2881 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     2941 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     3001 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     3061 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     3121 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     3181 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     3241 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     3301 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     3361 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     3421 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     3481 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     3541 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     3601 cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     3661 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     3721 gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     3781 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     3841 attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc
     3901 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     3961 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     4021 tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc
     4081 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     4141 ttctggaagg cgagcatcgt ttgttcgccc agcttctgta tggaacgggc atgcggatca
     4201 gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg atcatcgtgc
     4261 gggagggcaa gggctccaag gatcgggcct tgatgttacc cgagagcttg gcacccagcc
     4321 tgcgcgagca ggggaattaa ttcccacggg ttttgctgcc cgcaaacggg ctgttctggt
     4381 gttgctagtt tgttatcaga atcgcagatc cggcttcagc cggtttgccg gctgaaagcg
     4441 ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt
     4501 tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg
     4561 ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact
     4621 ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc
     4681 atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt
     4741 tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatgtaac ggtgaacagt
     4801 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     4861 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     4921 gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct
     4981 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     5041 tatgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     5101 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     5161 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     5221 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     5281 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     5341 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     5401 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     5461 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     5521 tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     5581 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     5641 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     5701 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     5761 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     5821 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     5881 tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac
     5941 ttgtaaattc tgctagaccc tctgtaaatt ccggtagacc tttgtgtgtt ttttttgttt
     6001 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     6061 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     6121 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     6181 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     6241 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     6301 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     6361 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     6421 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     6481 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     6541 aggcttaccc gtcttactgt cgg
//
