pLionII-miniPOMC vector (V004995)

MSDS

Basic Vector Information

Vector Name:: pLionII-miniPOMC

Antibiotic Resistance:: Ampicillin

Length:: 7284 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Hen G, Yosefi S, Shinder D, Or A, Mygdal S, Condiotti R, Galun E, Bor A, Sela-Donenfeld D, Friedman-Einat M.

Promoter:: CMV

pLionII-miniPOMC vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLionII-miniPOMC vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_28307        7284 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLionII-miniPOMC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Hen G, Yosefi S, Shinder D, Or A, Mygdal S, Condiotti R, Galun E, 
            Bor A, Sela-Donenfeld D, Friedman-Einat M.
  TITLE     Gene Transfer to Chicks Using Lentiviral Vectors Administered via 
            the Embryonic Chorioallantoic Membrane
  JOURNAL   PLoS ONE 7 (5), E36531 (2012)
  PUBMED    22606269
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Chen G, Sela-Donenfeld D, Yosefi S, Condiotti R, Friedman-Einat M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2011) Animal Science, ARO, Volcani Center, 30 
            Derech Hamacabim, Beit-Dagan 50250, Israel
REFERENCE   3  (bases 1 to 7284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "5"; pages: "E36531"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2011) Animal Science, ARO, Volcani Center, 30 Derech 
            Hamacabim, Beit-Dagan 50250, Israel"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1802..1906)
                     /label=AmpR promoter
     enhancer        2874..3253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      2954..3242
                     /label=CAG enhancer
                     /note="CAG enhancer"
                     /regulatory_class="enhancer"
     promoter        3255..3454
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4352..4370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4412..4791
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4792..4995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5040..5058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5041..5058
                     /label=T7 promoter
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     CDS             5163..5387
                     /codon_start=1
                     /gene="POMC"
                     /product="truncated pro-opiomelanocortin preproprotein"
                     /label=POMC
                     /note="mini POMC; derived from Homo sapiens"
                     /protein_id="AFC36445.1"
                     /translation="MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLE
                     CIRACKPREGKRSYSMEHFRWGKPVGKKR"
     gene            5163..5387
                     /gene="POMC"
                     /label=POMC
     misc_feature    6193..6781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(6787..6803)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7088..7104)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7112..7142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7157..7178)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."

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