LOCUS       40924_28307        7284 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLionII-miniPOMC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Hen G, Yosefi S, Shinder D, Or A, Mygdal S, Condiotti R, Galun E, 
            Bor A, Sela-Donenfeld D, Friedman-Einat M.
  TITLE     Gene Transfer to Chicks Using Lentiviral Vectors Administered via 
            the Embryonic Chorioallantoic Membrane
  JOURNAL   PLoS ONE 7 (5), E36531 (2012)
  PUBMED    22606269
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Chen G, Sela-Donenfeld D, Yosefi S, Condiotti R, Friedman-Einat M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2011) Animal Science, ARO, Volcani Center, 30 
            Derech Hamacabim, Beit-Dagan 50250, Israel
REFERENCE   3  (bases 1 to 7284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "5"; pages: "E36531"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2011) Animal Science, ARO, Volcani Center, 30 Derech 
            Hamacabim, Beit-Dagan 50250, Israel"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1802..1906)
                     /label=AmpR promoter
     enhancer        2874..3253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      2954..3242
                     /label=CAG enhancer
                     /note="CAG enhancer"
                     /regulatory_class="enhancer"
     promoter        3255..3454
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4352..4370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4412..4791
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4792..4995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5040..5058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5041..5058
                     /label=T7 promoter
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     CDS             5163..5387
                     /codon_start=1
                     /gene="POMC"
                     /product="truncated pro-opiomelanocortin preproprotein"
                     /label=POMC
                     /note="mini POMC; derived from Homo sapiens"
                     /protein_id="AFC36445.1"
                     /translation="MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLE
                     CIRACKPREGKRSYSMEHFRWGKPVGKKR"
     gene            5163..5387
                     /gene="POMC"
                     /label=POMC
     misc_feature    6193..6781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(6787..6803)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7088..7104)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7112..7142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7157..7178)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2161 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
     2221 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2281 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
     2341 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2401 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     2461 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggtaa
     2521 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2581 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2641 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2701 tttgctggcc ttttgctcac atggctcgac agatcttcaa tattggccat tagccatatt
     2761 attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     2821 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttggcattg
     2881 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2941 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3001 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3061 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3121 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3181 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     3241 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     3301 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3361 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     3421 taggcgtgta cggtgggagg tctatataag cagagctctg tgaaacttcg aggagtctct
     3481 ttgttgagga cttttgagtt ctcccttgag gctcccacag atacaataaa tatttgagat
     3541 tgaaccctgt cgagtatctg tgtaatcttt tttacctgtg aggtctcgga atccgggccg
     3601 agaacttcgc agttggcgcc cgaacaggga cttgattgag agtgattgag gaagtgaagc
     3661 tagagcaata gaaagctgtt aagcagaact cctgctgacc taaataggga agcagtagca
     3721 gacgctgcta acagtgagta tctctagtga agcggactcg agctcataat caagtcattg
     3781 tttaaaggcc cagataaatt acatctggtg actcttcgcg gaccttcaag ccaggagatt
     3841 cgccgaggga cagtcaacaa ggtaggagag attctacagc aacatgggga atggacaggg
     3901 gcgagattgg aaaatggcca ttaagagatg tagtaatgtt gctgtaggag taggggggaa
     3961 gagtaaaaaa tttggagaag ggaatttcag atgggccatt agaatggcta atgtatctac
     4021 aggacgagaa cctggtgata taccagagac tttagatcaa ctaaggttgg ttatttgcga
     4081 tttacaagaa agaagagaaa aatttggatc tagcaaagaa attgatatgg caattgtata
     4141 catgtggaat caggatatat atgggcacaa ataatttctc aagaaactgc tgactgtaca
     4201 gttaaagctg tcttacaatt gttgagtgct cataatgtta ctgaattaca aacagataat
     4261 ggaccaaatt ttaaaaatca aaagatggaa ggagtaattg tgacattaaa agtctttgcg
     4321 gtagcaggac ttttaaatat gacggcgcgc gtaatacgac tcactatagg gcgaattggg
     4381 taccgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     4441 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4501 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4561 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat
     4621 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     4681 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     4741 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     4801 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4861 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4921 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4981 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     5041 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg
     5101 agctcggatc cactagtaac ggccgccagt gtgctggaat tagcttgcgc gctgcctgga
     5161 agatgccgag atcgtgctgc agccgctcgg gggccctgtt gctggccttg ctgcttcagg
     5221 cctccatgga agtgcgtggc tggtgcctgg agagcagcca gtgtcaggac ctcaccacgg
     5281 aaagcaacct gctggagtgc atccgggcct gcaagccccg cgagggcaag cgctcctact
     5341 ccatggagca cttccgctgg ggcaagccgg tgggcaagaa gcggtaagga tccactagtc
     5401 cagtgtggtg gaattctgca gatatccagc acagtggcgg ccgctcgagt gcgccctaat
     5461 agaagctagt aataggatta aaagtagaag ctatggaaaa atttttgtat acagctttcg
     5521 ctatgcaaga attaggatgt aatcaaaatc aatttttctg caaaatccct cctgagttgt
     5581 ggacaaggta taatatgact ataaatcaaa caatatggaa tcatggaaat ataactttgg
     5641 gggaatggta taaccaaaca aaagatttac aacaaaagtt ttatgaaata ataatggaca
     5701 tagaacaaaa taatgtacaa gggaagaaag ggatacaaca attacaaaag tgggaagatt
     5761 gggtaggatg gataggaaat attccacaat atttaaaggg actattggga ggtatcttgg
     5821 gaataggatt aggagtgtta ttattgattt tatgtttacc tacattggtt gattgtataa
     5881 gaaattgtat ccacaagata ctaggataca cagtaattgc aatgcctgaa gtagaaggag
     5941 aagaaataca accacaaatg gaattgagga gaaatggtag gcaatgtggc atgtctgaaa
     6001 aagaggagga atgatgaagt atctcagact tattttataa gggagatact gtgctgagtt
     6061 cttccctttg aggaaggtat gtcatatgaa tccatttcga atcaaatcaa actaataaag
     6121 tatgtattgt aaggtaaaag gaaaagacaa agaagaagaa gaaagaagaa agccttcatc
     6181 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6241 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6301 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6361 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6421 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6481 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6541 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6601 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6661 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6721 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6781 cctgatcgat accgtcgacc tcgagggggg gccctcctaa aaaagaaaaa agggtggact
     6841 gggatgagta ttggaaccct gaaggcctta accagtgctt tgtgaaactt cgaggagtct
     6901 ctttgttgag gacttttgag ttctcccttg aggctcccac agatacaata aatatttgag
     6961 attgaaccct gtcgagtatc tgtgtaatct tttttacctg tgaggtctcg gaatccgggc
     7021 cgagaacttc gcactgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     7081 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7141 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7201 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7261 gaggcggttt gcgtattggg cgct
//
