pKT311 vector (V005171)

LOCUS       40924_27084        5952 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT311, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 5952)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      62..808
                     /note="pki; Trichoderma reesei pyruvate kinase I promoter; 
                     accession CP016233"
                     /regulatory_class="promoter"
     regulatory      215..224
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..1941
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     CDS             1981..2772
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2794..3523
                     /note="cbh2; Trichoderma reesei cellobiohydrolase II
                     terminator; accession CP016238"
                     /regulatory_class="terminator"
     promoter        complement(3590..3608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3866..4454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4628..5485)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5486..5590)
                     /label=AmpR promoter
     promoter        5936..5952
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"