LOCUS       40924_27084        5952 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT311, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 5952)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      62..808
                     /note="pki; Trichoderma reesei pyruvate kinase I promoter; 
                     accession CP016233"
                     /regulatory_class="promoter"
     regulatory      215..224
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..1941
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     CDS             1981..2772
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2794..3523
                     /note="cbh2; Trichoderma reesei cellobiohydrolase II
                     terminator; accession CP016238"
                     /regulatory_class="terminator"
     promoter        complement(3590..3608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3866..4454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4628..5485)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5486..5590)
                     /label=AmpR promoter
     promoter        5936..5952
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gcaggatcct taattaatcg aattctaggc cgcataggcc atactagtac
       61 tcgagataac ggtgagacta gcggccggtc cccttatccc agctgttcca cgttggcctg
      121 cccctcagtt agcgctcaac tcaatgcccc tcactggcga ggcgagggca aggatggagg
      181 ggcagcatcg cctgagttgg agcaaagcgg ccgggccgcc atgggagcag cgaaccaacg
      241 gagggatgcc gtgctttgtc gtggctgctg tggccaatcc gggcccttgg ttggctcaca
      301 gagcgttgct gtgagaccat gagctattat tgctaggtac agtatagaga gaggagagag
      361 agagagagag agagagaggg gaaaaaaggt gaggttgaag tgagaaaaaa aaaaaaaaaa
      421 aaaaatccaa ccactgacgg ctgccggctc tgccaccccc ctccctccac cccagaccac
      481 ctgcacactc agcgcgcagc atcacctaat cttggctcgc cttcccgcag ctcaggttgt
      541 tttttttttc tctctccctc gtcgaagccg cccttgttcc cttatttatt tccctctcca
      601 tccttgtctg cctttggtcc atctgcccct ttgtctgcat ctcttttgca cgcatcgcct
      661 tatcgtcgtc tcttttttca ctcacgggag cttgacgaag acctgactcg tgagcctcac
      721 ctgctgattt ctctcccccc ctcccgaccg gcttgacttt tgtttctcct ccagtacctt
      781 atcgcgaagc cggaagaacc tcttaacctc tagatggctt cgtacccctg ccatcaacac
      841 gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata acaaccgacg tacggcgttg
      901 cgccctcgcc ggcaacaaaa agccacggaa gtccgcctgg agcagaaaat gcccacgcta
      961 ctgcgggttt atatagacgg tccccacggg atggggaaaa ccaccaccac gcaactgctg
     1021 gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcgg
     1081 gtgttggggg cttccgagac aatcgcgaac atctacacca cacaacaccg cctcgaccag
     1141 ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc
     1201 atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc
     1261 tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc
     1321 ctgtgctacc cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg
     1381 ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg
     1441 gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg
     1501 gctatgctgg ccgcgattcg ccgcgtttat gggctgcttg ccaatacggt gcggtatctg
     1561 cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt cgggggcggc cgtgccgccc
     1621 cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt
     1681 accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtataa cgtgtttgcc
     1741 tgggctttgg acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac
     1801 gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag
     1861 acccacgtca ccaccccagg ctccataccg acgatctgcg acctggcgcg cacgtttgcc
     1921 cgggagatgg gggaggctaa cgaatgcagt ggaggaggtt ctggaaggag gatatgcata
     1981 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     2041 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     2101 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     2161 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     2221 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     2281 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     2341 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     2401 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     2461 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     2521 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     2581 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     2641 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     2701 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     2761 gacgagttct tctgaggtac cgagctcgaa tttggctttc gtgaccgggc ttcaaacaat
     2821 gatgtgcgat ggtgtggttc ccggttggcg gagtctttgt ctactttggt tgtctgtcgc
     2881 aggtcggtag accgcaaatg agcaactgat ggattgttgc cagcgatact ataattcaca
     2941 tggatggtct ttgtcgatca gtagctagtg agagagagag aacatctatc cacaatgtcg
     3001 agtgtctatt agacatactc cgagaataaa gtcaactgtg tctgtgatct aaagatcgat
     3061 tcggcagtcg agtagcgtat aacaactccg agtaccagcg aaagcacgtc gtgacaggag
     3121 cagggctttg ccaactgcgc aaccttgctt gaatgaggat acacggggtg caacatggct
     3181 gtactgatcc atcgcaacca aaatttctgt ttatagatca agctggtaga ttccaattac
     3241 tccacctctt gcgcttctcc atgacatgta agtgcacgtg gaaaccatac ccaaattgcc
     3301 tacagctgcg gagcatgagc ctatggcgat cagtctggtc atgttaacca gcctgtgctc
     3361 tgacgttaat gcagaataga aagccgcggt tgcaatgcaa atgatgatgc ctttgcagaa
     3421 atggcttgct cgctgactga taccagtaac aactttgctt ggccgtctag cgctgttgat
     3481 tgtattcatc acaacctcgt ctccctcctt tgggttgagc tctcgagatg tcgactgatt
     3541 taaatcatga agcttaaagt ttaaacgcgg ccgcggatct gccggtctcc ctatagtgag
     3601 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     3661 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5581 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5641 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     5701 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5761 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5821 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     5881 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     5941 ggtgacacta ta
//
