Basic Vector Information
LITMUS29 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
LITMUS29 vector Sequence
LOCUS LITMUS29. 2820 bp DNA circular SYN 01-JAN-1980 DEFINITION High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS28. ACCESSION . VERSION . KEYWORDS LITMUS29. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2820) AUTHORS Evans PD, Cook SN, Riggs PD, Noren CJ. TITLE LITMUS: multipurpose cloning vectors with a novel system for bidirectional in vitro transcription. JOURNAL BioTechniques 1995;19:130-5. PUBMED 7669286 REFERENCE 2 (bases 1 to 2820) AUTHORS New England Biolabs TITLE Direct Submission REFERENCE 3 (bases 1 to 2820) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "1995"; volume: "19"; pages: "130-5" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..2820 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 38..142 /label=AmpR promoter CDS 143..1000 /label=AmpR /note="beta-lactamase" rep_origin complement(1045..1554) /direction=LEFT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin 1665..2253 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2314..2335 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2350..2380 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2388..2404 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2412..2428 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2449..2466 /gene="lacZalpha" /label=lacZalpha promoter /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" misc_feature 2460..2605 /gene="lacZalpha" /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(2600..2617) /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2621..2637) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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