LOCUS       LITMUS29.        2820 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High copy-number E. coli vector with a multiple cloning site flanked
            by mutant T7 promoters. The MCS is reversed relative to LITMUS28.
ACCESSION   .
VERSION     .
KEYWORDS    LITMUS29
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Evans PD, Cook SN, Riggs PD, Noren CJ.
  TITLE     LITMUS: multipurpose cloning vectors with a novel system for 
            bidirectional in vitro transcription.
  JOURNAL   BioTechniques 1995;19:130-5.
  PUBMED    7669286
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1995"; volume: "19"; pages: "130-5"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /label=AmpR promoter
     CDS             143..1000
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1665..2253
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2350..2380
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2449..2466
                     /gene="lacZalpha"
                     /label=lacZalpha promoter
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     misc_feature    2460..2605
                     /gene="lacZalpha"
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2600..2617)
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     primer_bind     complement(2621..2637)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
      361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga
     1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc
     2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca
     2461 ctagtgggta ccagagctcc ctaggttcta gaaccggtga cgtctcccat ggtgaagctt
     2521 ggatccacga tatcctgcag gaattcctcg agaccgtacg tgcgcgcgaa tgcatccaga
     2581 tcttccctct agtcaaggcc ttaagtgagt cgtattacgg actggccgtc gttttacaac
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2761 gcctgaatgg cgaatggcgc ttcgcttggt aataaagccc gcttcggcgg gctttttttt
//
