pANT7_cGST vector (V012553)

LOCUS       pANT7_cGST.        5964 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for in vitro transcription and 
            translation of a protein with a C-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pANT7_cGST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau 
            AY, Walter JC, LaBaer J.
  TITLE     Self-assembling protein microarrays.
  JOURNAL   Science 2004;305:86-90.
  PUBMED    15232106
REFERENCE   2  (bases 1 to 5964)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004"; volume: "305"; pages: "86-90"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..514
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             516..518
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     protein_bind    549..673
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        698..728
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             782..1438
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1783..2085
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2129..2253)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2276..2929
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     terminator      3020..3067
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4029..4886)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4887..4991)
                     /label=AmpR promoter
     rep_origin      complement(5325..5780)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5925..5941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5948..5964
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"