LOCUS       pANT7_cGST.        5964 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for in vitro transcription and 
            translation of a protein with a C-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pANT7_cGST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau 
            AY, Walter JC, LaBaer J.
  TITLE     Self-assembling protein microarrays.
  JOURNAL   Science 2004;305:86-90.
  PUBMED    15232106
REFERENCE   2  (bases 1 to 5964)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004"; volume: "305"; pages: "86-90"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..514
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             516..518
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     protein_bind    549..673
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        698..728
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             782..1438
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1783..2085
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2129..2253)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2276..2929
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     terminator      3020..3067
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4029..4886)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4887..4991)
                     /label=AmpR promoter
     rep_origin      complement(5325..5780)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5925..5941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5948..5964
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
       61 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
      121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
      181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
      241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
      301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc gtattcaaca
      361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
      421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
      481 ggacgtggtt ttcctttgaa aaacacgatg ataatatgga tcggatccga attcgagctc
      541 cgtcatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      601 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      661 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      721 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      781 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      841 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      901 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      961 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1021 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1081 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1141 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1201 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1261 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1321 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
     1381 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1441 aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1501 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct
     1561 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1621 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1681 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1741 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2161 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2221 cgtttctcgt tcagctttct tgtacaaagt ggttgatgac aagcttgcgg ccgcactcga
     2281 gcctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga
     2341 atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg
     2401 aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga
     2461 tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt
     2521 gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat
     2581 tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt
     2641 tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata
     2701 tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt
     2761 tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg
     2821 tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc
     2881 tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctggt
     2941 tccgcgttga agatctgact gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agtttaaaca
     3001 ctagtccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     3061 ttttttgctg aaaggaggaa ctatatccgg gcttcctcgc tcactgactc gctgcgctcg
     3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     5101 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5161 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5221 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
     5281 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa
     5341 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5401 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5461 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5521 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5581 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5641 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5701 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5761 tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
     5821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5941 tgaattgtaa tacgactcac tata
//
