pLEXX-AK vector (V012453)

LOCUS       pLEXX-AK.        2891 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for simultaneous cloning of two DNA fragments in 
            the ClonePlex(R) library construction system.
ACCESSION   .
VERSION     .
KEYWORDS    pLEXX-AK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To prepare the vector for cloning, linearize at the HincII sites and
            dephosphorylate.
FEATURES             Location/Qualifiers
     source          1..2891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      65..96
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        97..199
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             200..1057
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1084..1111
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1123..1710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1732..1761)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             1984..2796
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      2801..2846
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"