LOCUS       pLEXX-AK.        2891 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for simultaneous cloning of two DNA fragments in 
            the ClonePlex(R) library construction system.
ACCESSION   .
VERSION     .
KEYWORDS    pLEXX-AK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To prepare the vector for cloning, linearize at the HincII sites and
            dephosphorylate.
FEATURES             Location/Qualifiers
     source          1..2891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      65..96
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        97..199
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             200..1057
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1084..1111
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1123..1710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1732..1761)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             1984..2796
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      2801..2846
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc
       61 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      121 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      181 aggagctaag gaagctaaaa tgagtattca acatttccgt gtcgccctta ttcccttttt
      241 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      301 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      361 ccttgagagt ttacgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      421 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      481 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc tcacggatgg
      541 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      601 cttacttctg gcaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      661 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      721 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      781 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      841 tgcaggatca cttctgcgct cggccctccc ggctggctgg tttattgctg ataaatctgg
      901 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      961 ccgcatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1021 gatcgctgag ataggtgcct cactgattaa gcattggtaa tgagggccca aatgtaatca
     1081 cctggctcac cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc
     1141 ccctgacgag catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact
     1201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1561 aagaacagta tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt
     1621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag
     1741 gcccacccgt gaaggtgagc cagtgagttg attgcagtcc agttacgctg gagtctgagg
     1801 ctcgtcctga atgatatcaa gcttgaattc gttgacgcat atctggatcc tgcagccgat
     1861 acggtcgtcg tccgtttaaa cgttgtgtct caaaatctct gatgtcacgt tgcacaagat
     1921 aaaaatatat catcatgaac aataaaaccg tctgcttaca taaacagtaa tacaaggggt
     1981 gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg
     2041 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2101 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2161 agcgttgcca atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg
     2221 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2281 gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2341 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2401 ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2461 ttggttggtg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2521 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2581 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2641 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2701 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2761 aaattgcagt ttcacttgat gctcgatgag tttttctaat caaataaaac gaaaggctca
     2821 gtcgaaagac tgagcctttc gttttaatct ggaaaaacca ccctggcgct gcaggttcca
     2881 gattcctggt t
//
