pMiniT vector (V012443)

LOCUS       pMiniT.        2525 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact bacterial vector that employs a toxic minigene for 
            high-efficiency cloning of PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pMiniT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..251
                     /gene="tnpA"
                     /label=tnpA promoter
                     /note="tnpA promoter"
                     /note="promoter of the IS10 transposase"
     CDS             252..482
                     /codon_start=1
                     /product="transposase encoded by the IS10 tnpA gene"
                     /label=transposase encoded by the IS10 tnpA gene
                     /note="tnpA transposase"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAV"
     RBS             504..510
                     /label=Shine-Dalgarno sequence
                     /note="Shine-Dalgarno sequence"
                     /note="ribosome binding site"
     CDS             518..523
                     /codon_start=1
                     /product="two-residue polypeptide that poisons the E. coli 
                     translation machinery"
                     /label=two-residue polypeptide that poisons the E. col
                     /note="toxic minigene"
                     /translation="MI"
     misc_feature    524..535
                     /label=stop codons
                     /note="stop codons"
     promoter        565..669
                     /label=AmpR promoter
     CDS             670..1527
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1701..2289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"