LOCUS       pMiniT.        2525 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact bacterial vector that employs a toxic minigene for 
            high-efficiency cloning of PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pMiniT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..251
                     /gene="tnpA"
                     /label=tnpA promoter
                     /note="tnpA promoter"
                     /note="promoter of the IS10 transposase"
     CDS             252..482
                     /codon_start=1
                     /product="transposase encoded by the IS10 tnpA gene"
                     /label=transposase encoded by the IS10 tnpA gene
                     /note="tnpA transposase"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAV"
     RBS             504..510
                     /label=Shine-Dalgarno sequence
                     /note="Shine-Dalgarno sequence"
                     /note="ribosome binding site"
     CDS             518..523
                     /codon_start=1
                     /product="two-residue polypeptide that poisons the E. coli 
                     translation machinery"
                     /label=two-residue polypeptide that poisons the E. col
                     /note="toxic minigene"
                     /translation="MI"
     misc_feature    524..535
                     /label=stop codons
                     /note="stop codons"
     promoter        565..669
                     /label=AmpR promoter
     CDS             670..1527
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1701..2289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccggttcaga caggataaag aggaacgcag aatgttagac aacacccgct tacgcatagc
       61 tattcagaaa tcaggccgtt taagcgatga ttcacgagaa ttgctggccc gctgcggcat
      121 aaaaattaat ttacacactc agcgctgatg aatcccctaa tgattttggt aaaaatcatt
      181 aagttaaggt ggacacacat cttgtcatat gattaaatgg tttcgcgaaa aatcaataat
      241 cagacaacaa gatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg
      301 aattacactt aaaacgactc aacagcttaa cgttggcttg ccacgcatta cttgactgta
      361 aaactctcac tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata
      421 acatcaaacg aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg
      481 tatcgctcga gggatccgaa ttcaggaggt aaaaaccatg atctgataat aatgacgtca
      541 gaattctcga gtcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1381 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2281 ctatggaaaa acgccagcaa tgcggccttt ttacggttcc tggccttttg ctggcctttt
     2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2521 tgcag
//
