pT7Blue-3 vector (V012385)

LOCUS       pT7Blue-3.        3821 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a T7 promoter and dual ampicillin/kanamycin 
            resistance, and with a multiple cloning site that includes an EcoRV 
            site flanked by EcoRI sites.
ACCESSION   .
VERSION     .
KEYWORDS    pT7Blue-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    51..178
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(186..202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(343..798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        825..929
                     /label=AmpR promoter
     CDS             930..1787
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             1936..2748
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2836..3424
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3712..3733
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3748..3778
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3786..3802
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."