LOCUS       pT7Blue-3.        3821 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a T7 promoter and dual ampicillin/kanamycin 
            resistance, and with a multiple cloning site that includes an EcoRV 
            site flanked by EcoRI sites.
ACCESSION   .
VERSION     .
KEYWORDS    pT7Blue-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    51..178
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(186..202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(343..798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        825..929
                     /label=AmpR promoter
     CDS             930..1787
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             1936..2748
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2836..3424
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3712..3733
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3748..3778
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3786..3802
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctc ggtaccacgc
       61 atgctgcaga cgcgttacgt atcggatcca gaattcgtga tatctgaatt cgtcgacaag
      121 cttctcgagc ctaggctagc tctagaccac acgtgtgggg gcccgagctc gcggccgcac
      181 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      241 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      301 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata
      361 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
      421 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
      481 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
      541 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
      601 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
      661 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
      721 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
      781 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1861 aggtgaagat cctttttgat aatctcatga acaataaaac tgtctgctta cataaacagt
     1921 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta
     1981 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2041 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2101 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2161 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2221 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
     2281 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2341 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2401 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2461 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2521 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2581 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2641 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2701 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt
     2761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2941 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3661 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     3721 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     3781 tggaattgtg agcggataac aatttcacac aggaaacagc t
//
