pT7Blue vector (V012384)

LOCUS       pT7Blue.        2887 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a T7 promoter, and with a modified multiple 
            cloning site that includes an EcoRV site for blunt cloning or TA 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pT7Blue
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    45..125
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(126..142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(284..739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        766..870
                     /label=AmpR promoter
     CDS             871..1728
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1902..2490
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2778..2799
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2814..2844
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2852..2868
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."