LOCUS       pT7Blue.        2887 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a T7 promoter, and with a modified multiple 
            cloning site that includes an EcoRV site for blunt cloning or TA 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pT7Blue
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    45..125
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(126..142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(284..739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        766..870
                     /label=AmpR promoter
     CDS             871..1728
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1902..2490
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2778..2799
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2814..2844
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2852..2868
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctt gcatgcctgc
       61 aggtcgactc tagaggatct actagtcata tggatatcgg atccccgggt accgagctcg
      121 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      181 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      241 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggaaattgt aaacgttaat
      301 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      361 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
      421 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
      481 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
      541 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
      601 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
      661 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
      721 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2041 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     2821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     2881 aacagct
//
