Basic Vector Information
- Vector Name:
- pUC18
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2686 bp
- Type:
- Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Yanisch-Perron C, Vieira J, Messing J.
- Copy Number:
- High copy number
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pUC18 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC18 vector Sequence
LOCUS 40924_45023 2686 bp DNA circular SYN 01-JAN-1980 DEFINITION Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2686) AUTHORS Yanisch-Perron C, Vieira J, Messing J. TITLE Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. JOURNAL Gene 1985;33:103-19. PUBMED 2985470 REFERENCE 2 (bases 1 to 2686) TITLE Direct Submission REFERENCE 3 (bases 1 to 2686) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1985"; volume: "33"; pages: "103-19" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..2686 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(399..455) /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(465..481) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(489..505) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(513..543) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(558..579) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(867..1455) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1629..2486) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2487..2591) /label=AmpR promoter
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