LOCUS       Exported                2686 bp DNA     circular SYN 30-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(770..2686,1..769)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    191..212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        227..257
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     289..305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             301..624
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    315..371
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(375..391)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        865..969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             970..1830
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2001..2589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
       61 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      301 atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
      361 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      421 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      481 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      541 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      601 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      661 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      721 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      781 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1201 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1501 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataacc
//
