Basic Vector Information
pTurboFP602-B vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTurboFP602-B vector Sequence
LOCUS pTurboFP602-B. 4115 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing TurboFP602 in bacteria. ACCESSION . VERSION . KEYWORDS pTurboFP602-B. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4115) AUTHORS Evrogen TITLE Direct Submission REFERENCE 2 (bases 1 to 4115) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4115 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 115..117 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 133..837 /label=TurboFP602 /note="red-shifted derivative of red fluorescent protein from Entacmaea quadricolor" terminator 861..955 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 999..1655 /label=CmR /note="chloramphenicol acetyltransferase" terminator 1723..1809 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1965..2105 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(2291..2879) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3053..3910) /label=AmpR /note="beta-lactamase" promoter complement(3911..4015) /label=AmpR promoter
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