LOCUS       pTurboFP602-B.        4115 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboFP602 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboFP602-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..837
                     /label=TurboFP602
                     /note="red-shifted derivative of red fluorescent protein
                     from Entacmaea quadricolor"
     terminator      861..955
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             999..1655
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1723..1809
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1965..2105
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2291..2879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3053..3910)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3911..4015)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aactatgaga
      121 ggatcgggat ccatggtggg tgaggatagc gagctgatca ccgagaacat gcacatgaaa
      181 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
      241 aagccctacg agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc
      301 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac
      361 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
      421 atcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccagaac
      481 ggctgcctca tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg
      541 atgcagaaga aaacactcgg ctgggaggcc agcaccgaga tgctgtaccc cgctgacagc
      601 ggcctgagag gccatggcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc
      661 tccctcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc
      721 cacttcgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
      781 cagcacgaga tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagctga
      841 agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      901 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      961 ctagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1021 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1081 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1141 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1201 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1261 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1321 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1381 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1441 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1501 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1561 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     1621 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     1681 gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct
     1741 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1801 aggacaaatc cgccgctcta gagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     1861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     1981 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     2041 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2101 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtctgt cggctgcggc
     2161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2461 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     2521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2701 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2941 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3001 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3061 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gctgcctgac
     3121 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3181 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3241 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3301 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3361 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3421 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3481 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3541 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3601 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3661 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3721 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3781 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3841 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3901 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3961 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4021 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4081 aaaataggcg tatcacgagg ccctttcgtc ttcac
//
